A probable link between the DedA protein and resistance to selenite

Fouzia Ledgham; Benjamin Quest; Tatiana Vallaeys; Max Mergeay; Jacques Covès

doi:10.1016/j.resmic.2004.11.003

A probable link between the DedA protein and resistance to selenite

Fouzia Ledgham, Benjamin Quest, Tatiana Vallaeys, Max Mergeay, Jacques Covès

Research output › peer-review

Abstract

To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while the MIC for the wild-type strain was estimated as 4-6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.

Original language	English
Pages (from-to)	367-374
Number of pages	8
Journal	Research in Microbiology
Volume	156
Issue number	3
DOIs	https://doi.org/10.1016/j.resmic.2004.11.003
State	Published - Apr 2005

ASJC Scopus subject areas

Microbiology
Molecular Biology

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Cite this

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title = "A probable link between the DedA protein and resistance to selenite",

abstract = "To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while the MIC for the wild-type strain was estimated as 4-6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.",

keywords = "Selenium oxyanions, Selenite, Resistance, Random mutagenesis, DedA, Ralstonia metallidurans CH34",

author = "Fouzia Ledgham and Benjamin Quest and Tatiana Vallaeys and Max Mergeay and Jacques Cov{\`e}s",

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T1 - A probable link between the DedA protein and resistance to selenite

AU - Ledgham, Fouzia

AU - Quest, Benjamin

AU - Vallaeys, Tatiana

AU - Mergeay, Max

AU - Covès, Jacques

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N2 - To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while the MIC for the wild-type strain was estimated as 4-6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.

AB - To understand the molecular events involved in the reduction of selenite to non-toxic elemental selenium, 4000 clones of Ralstonia metallidurans CH34 were produced by random Tn5 transposon integration mutagenesis. Eight mutants were able to resist up to 15 mM selenite while the MIC for the wild-type strain was estimated as 4-6 mM selenite. The identification of the disrupted genes was carried out by Southern blot analysis and inverse PCR. The three resistant mutants containing only one insertion were further characterized. Tn5 disrupted a gene that encoded a protein which was closely related to proteins of the DedA family. This family represents a group of integral membrane proteins with completely unknown functions. Phenotypic characterization of the dedA mutants and selenite consumption experiments strongly suggest that DedA is involved in the uptake of selenite.

KW - Selenium oxyanions

KW - Selenite

KW - Resistance

KW - Random mutagenesis

KW - DedA

KW - Ralstonia metallidurans CH34

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U2 - 10.1016/j.resmic.2004.11.003

DO - 10.1016/j.resmic.2004.11.003

M3 - Article

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JO - Research in Microbiology

JF - Research in Microbiology

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