An ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat

H. Reyners; E. Gianfelici de Reyners; J. M. Jadin; J. R. Maisin

doi:10.1007/BF00223232

An ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat

H. Reyners, E. Gianfelici de Reyners, J. M. Jadin, J. R. Maisin

Research output › peer-review

Abstract

A quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. The method involves the counting of labeled "caveolae intracellulares" in endothelial cells with the use of horseradish peroxidase as a tracer molecule. A mean pinocytotic value per capillary is then computed. It is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. This information may provide an ultrastructural quantitative approach to the analysis of the blood brain barrier and of other problems related to vascular permeability of the cerebral cortex.

Original language	English
Pages (from-to)	93-99
Number of pages	7
Journal	Cell and Tissue Research
Volume	157
Issue number	1
DOIs	https://doi.org/10.1007/BF00223232
State	Published - Mar 1975

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology
Cell Biology

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10.1007/BF00223232

Cite this

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title = "An ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat",

abstract = "A quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. The method involves the counting of labeled {"}caveolae intracellulares{"} in endothelial cells with the use of horseradish peroxidase as a tracer molecule. A mean pinocytotic value per capillary is then computed. It is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. This information may provide an ultrastructural quantitative approach to the analysis of the blood brain barrier and of other problems related to vascular permeability of the cerebral cortex.",

keywords = "Blood brain barrier, Pinocytosis, X irradiation",

author = "H. Reyners and {de Reyners}, {E. Gianfelici} and Jadin, {J. M.} and Maisin, {J. R.}",

year = "1975",

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doi = "10.1007/BF00223232",

language = "English",

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T1 - An ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat

AU - Reyners, H.

AU - de Reyners, E. Gianfelici

AU - Jadin, J. M.

AU - Maisin, J. R.

PY - 1975/3

Y1 - 1975/3

N2 - A quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. The method involves the counting of labeled "caveolae intracellulares" in endothelial cells with the use of horseradish peroxidase as a tracer molecule. A mean pinocytotic value per capillary is then computed. It is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. This information may provide an ultrastructural quantitative approach to the analysis of the blood brain barrier and of other problems related to vascular permeability of the cerebral cortex.

AB - A quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. The method involves the counting of labeled "caveolae intracellulares" in endothelial cells with the use of horseradish peroxidase as a tracer molecule. A mean pinocytotic value per capillary is then computed. It is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. This information may provide an ultrastructural quantitative approach to the analysis of the blood brain barrier and of other problems related to vascular permeability of the cerebral cortex.

KW - Blood brain barrier

KW - Pinocytosis

KW - X irradiation

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U2 - 10.1007/BF00223232

DO - 10.1007/BF00223232

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AN - SCOPUS:0016663162

SN - 0302-766X

VL - 157

SP - 93

EP - 99

JO - Cell and Tissue Research

JF - Cell and Tissue Research

IS - 1

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