The B54X position of the 8 MW RA3 research reactor at the Ezeiza Atomic Centre of the Argentine National Atomic Energy Commission is currently being used for NAA irradiations. The facility with a nominal average fluence of 5 9 1013 cm-2 s-1 is dedicated to long irradiations of up to 5 h. Samples are being measured after a decay of typically 7 and 30 days. With the aim of implementing the k0-NAA method at the Nuclear Analytical Techniques Laboratory of the Centre, the reactor parameters a and f were estimated applying multi monitor methods using the Kayzero for Windows software. After a careful recalibration of the HPGe detector, SMELS III, NIST SRM 1633b and several other matrix RM’s were analyzed using the k0 standardization in order to verify the proper implementation of the k0-NAA approach. The found accuracy and associated uncertainties are discussed. In general, good agreement was obtained between results of this work and the reference values of the individual reference materials, thus proving successful first implementation of the above method and trueness of the results achieved. The obtained detection limits for several elements were evaluated.