Chapter 30: Site-specific labeling of his-tagged Nanobodies with 99mTc: a practical guide

Catarina Xavier; Nick Devoogdt; Sophie Hernot; Ilse Vaneycken; Matthias D'huyvetter; Jens De Vos; Sam Massa; Tony Lahoutte; Vicky Caveliers; An Aerts; Nathalie Impens; Sarah Baatout

Chapter 30: Site-specific labeling of his-tagged Nanobodies with 99mTc: a practical guide

Catarina Xavier, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D'huyvetter, Jens De Vos, Sam Massa, Tony Lahoutte, Vicky Caveliers, An Aerts, Nathalie Impens, Sarah Baatout

Research output › peer-review

Abstract

Chapter 30: the 99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.

Original language	English
Title of host publication	Singe Domain Antibodies, Methods and Protocols
Place of Publication	Springer New York Heidelberg Dordrecht London, United States
Publisher	Springer
Pages	485-490
Edition	1
ISBN (Print)	978-1-61779-968-6
State	Published - Jan 2012

Publication series

Name	Methods in Molecular Biology
Number	911

Cite this

Xavier, C., Devoogdt, N., Hernot, S., Vaneycken, I., D'huyvetter, M., De Vos, J., Massa, S., Lahoutte, T., Caveliers, V., Aerts, A., Impens, N., & Baatout, S. (2012). Chapter 30: Site-specific labeling of his-tagged Nanobodies with 99mTc: a practical guide. In Singe Domain Antibodies, Methods and Protocols (1 ed., pp. 485-490). (Methods in Molecular Biology; No. 911). Springer.

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abstract = "Chapter 30: the 99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.",

keywords = "Nanobody, 99mTc",

author = "Catarina Xavier and Nick Devoogdt and Sophie Hernot and Ilse Vaneycken and Matthias D'huyvetter and {De Vos}, Jens and Sam Massa and Tony Lahoutte and Vicky Caveliers and An Aerts and Nathalie Impens and Sarah Baatout",

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Xavier, C, Devoogdt, N, Hernot, S, Vaneycken, I, D'huyvetter, M, De Vos, J, Massa, S, Lahoutte, T, Caveliers, V, Aerts, A, Impens, N & Baatout, S 2012, Chapter 30: Site-specific labeling of his-tagged Nanobodies with 99mTc: a practical guide. in Singe Domain Antibodies, Methods and Protocols. 1 edn, Methods in Molecular Biology, no. 911, Springer, Springer New York Heidelberg Dordrecht London, United States, pp. 485-490.

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