Chapter 30: Site-specific labeling of his-tagged Nanobodies with 99mTc: a practical guide

Catarina Xavier, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D'huyvetter, Jens De Vos, Sam Massa, Tony Lahoutte, Vicky Caveliers, An Aerts, Nathalie Impens, Sarah Baatout

    Research outputpeer-review


    Chapter 30: the 99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.
    Original languageEnglish
    Title of host publicationSinge Domain Antibodies, Methods and Protocols
    Place of PublicationSpringer New York Heidelberg Dordrecht London, United States
    ISBN (Print)978-1-61779-968-6
    StatePublished - Jan 2012

    Publication series

    NameMethods in Molecular Biology

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