TY - JOUR
T1 - Characterization of a temperate phage hosted by Alcaligenes eutrophus strain A5
AU - Faelen, M.
AU - Merlin, C.
AU - Geuskens, Maurice
AU - Mergeay, M.
AU - Toussaint, A.
PY - 1993
Y1 - 1993
N2 - Nineteen strains of Alcaligenes eutrophus were tested for the presence of prophages. One strain that lysed upon mitomycin C treatment produced a phage which could not form plaques on any of the strains available. DNA extracted from partially purified phage lysates was digested with various restriction enzymes which showed that the 42 kb long viral double-stranded DNA circularizes by means of cohesive ends. To our knowledge, this is the first description of a phage for the genus Alcaligenes.
AB - Nineteen strains of Alcaligenes eutrophus were tested for the presence of prophages. One strain that lysed upon mitomycin C treatment produced a phage which could not form plaques on any of the strains available. DNA extracted from partially purified phage lysates was digested with various restriction enzymes which showed that the 42 kb long viral double-stranded DNA circularizes by means of cohesive ends. To our knowledge, this is the first description of a phage for the genus Alcaligenes.
KW - Bacteriophage, Prophage, Alcaligenes eutrophus
KW - Mitomycin C, Characterization
UR - http://www.scopus.com/inward/record.url?scp=0027751632&partnerID=8YFLogxK
U2 - 10.1016/0923-2508(93)90065-A
DO - 10.1016/0923-2508(93)90065-A
M3 - Article
C2 - 8140281
AN - SCOPUS:0027751632
SN - 0923-2508
VL - 144
SP - 627
EP - 631
JO - Research in Microbiology
JF - Research in Microbiology
IS - 8
ER -