Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae

Sahaya Glingston Rajakani; Britto Xavier; Ngoc Minh Nguyen; Qiang Lin; Nienke L. Plantin; Bastiaan H.J. Wittekamp; Olympia Zarkotou; Rob Van Houdt; Youri Glupczynski; Spyros Pournaras; Marc J.M. Bonten; Surbhi Malhotra-Kumar

doi:10.1128/aac.01574-24

Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae

Sahaya Glingston Rajakani, Britto Xavier, Ngoc Minh Nguyen, Qiang Lin, Nienke L. Plantin, Bastiaan H.J. Wittekamp, Olympia Zarkotou, Rob Van Houdt, Youri Glupczynski, Spyros Pournaras, Marc J.M. Bonten, Surbhi Malhotra-Kumar

Microbiology

Research output › peer-review

Abstract

Colistin is one of the last-line antibiotics against multi-drug-resistant (MDR) gram-negative pathogens, such as Klebsiella pneumoniae. Using long-read sequencing, we observed remarkable genome-wide transposition events in MDR K. pneumoniae exposed to colistin in patients receiving treatment for respiratory infections or as part of selective decolonization strategies and further confirmed these on in vitro selection experiments. These data add yet another dimension to the role of antibiotics in mediating specific processes in bacteria beyond antibiotic resistance.

Original language	English
Number of pages	10
Journal	Antimicrobial Agents and Chemotherapy
DOIs	https://doi.org/10.1128/aac.01574-24
State	Published - May 2025

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Glingston Rajakani, S., Xavier, B., Nguyen, N. M., Lin, Q., Plantin, N. L., Wittekamp, B. H. J., Zarkotou, O., Van Houdt, R., Glupczynski, Y., Pournaras, S., Bonten, M. J. M., & Malhotra-Kumar, S. (2025). Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.01574-24

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title = "Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae",

abstract = "Colistin is one of the last-line antibiotics against multi-drug-resistant (MDR) gram-negative pathogens, such as Klebsiella pneumoniae. Using long-read sequencing, we observed remarkable genome-wide transposition events in MDR K. pneumoniae exposed to colistin in patients receiving treatment for respiratory infections or as part of selective decolonization strategies and further confirmed these on in vitro selection experiments. These data add yet another dimension to the role of antibiotics in mediating specific processes in bacteria beyond antibiotic resistance.",

keywords = "Antibiotic resistance, Insertion sequence elements, Colistin, Muti-drug resistant Klebsiella penumoniae",

author = "{Glingston Rajakani}, Sahaya and Britto Xavier and Nguyen, {Ngoc Minh} and Qiang Lin and Plantin, {Nienke L.} and Wittekamp, {Bastiaan H.J.} and Olympia Zarkotou and {Van Houdt}, Rob and Youri Glupczynski and Spyros Pournaras and Bonten, {Marc J.M.} and Surbhi Malhotra-Kumar",

note = "Score=10 This study is registered with ClinicalTrials.gov as NCT02208154",

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doi = "10.1128/aac.01574-24",

language = "English",

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Glingston Rajakani, S, Xavier, B, Nguyen, NM, Lin, Q, Plantin, NL, Wittekamp, BHJ, Zarkotou, O, Van Houdt, R, Glupczynski, Y, Pournaras, S, Bonten, MJM & Malhotra-Kumar, S 2025, 'Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae', Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.01574-24

TY - JOUR

T1 - Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae

AU - Glingston Rajakani, Sahaya

AU - Xavier, Britto

AU - Nguyen, Ngoc Minh

AU - Lin, Qiang

AU - Plantin, Nienke L.

AU - Wittekamp, Bastiaan H.J.

AU - Zarkotou, Olympia

AU - Van Houdt, Rob

AU - Glupczynski, Youri

AU - Pournaras, Spyros

AU - Bonten, Marc J.M.

AU - Malhotra-Kumar, Surbhi

N1 - Score=10 This study is registered with ClinicalTrials.gov as NCT02208154

PY - 2025/5

Y1 - 2025/5

N2 - Colistin is one of the last-line antibiotics against multi-drug-resistant (MDR) gram-negative pathogens, such as Klebsiella pneumoniae. Using long-read sequencing, we observed remarkable genome-wide transposition events in MDR K. pneumoniae exposed to colistin in patients receiving treatment for respiratory infections or as part of selective decolonization strategies and further confirmed these on in vitro selection experiments. These data add yet another dimension to the role of antibiotics in mediating specific processes in bacteria beyond antibiotic resistance.

AB - Colistin is one of the last-line antibiotics against multi-drug-resistant (MDR) gram-negative pathogens, such as Klebsiella pneumoniae. Using long-read sequencing, we observed remarkable genome-wide transposition events in MDR K. pneumoniae exposed to colistin in patients receiving treatment for respiratory infections or as part of selective decolonization strategies and further confirmed these on in vitro selection experiments. These data add yet another dimension to the role of antibiotics in mediating specific processes in bacteria beyond antibiotic resistance.

KW - Antibiotic resistance

KW - Insertion sequence elements

KW - Colistin

KW - Muti-drug resistant Klebsiella penumoniae

U2 - 10.1128/aac.01574-24

DO - 10.1128/aac.01574-24

M3 - Article

SN - 0066-4804

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

ER -