Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.

Rughia Idris; Melanie Kuffner; Levente Bodrossy; Markus Puschenreiter; Sébastien Monchy; Walter Wenzel; Angela Sessitsch; Max Mergeay

doi:10.1016/j.syapm.2006.01.011

Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.

Rughia Idris, Melanie Kuffner, Levente Bodrossy, Markus Puschenreiter, Sébastien Monchy, Walter Wenzel, Angela Sessitsch, Max Mergeay

Research output › peer-review

Abstract

Various pink-pigmented, facultative methylotrophic (PPFM) bacteria (strains iEII3, iEIV1, iEI6, iEII1, iEIII3 iEIII4, iEIII5, iRII1, iRII2, iRIII1, iRIV1 and iRIV2) were obtained from the rhizosphere and endosphere of hyperaccumulating plant Thlaspi goesingense grown in Redschlag, Austria 16. Due to their unexpected diversity, abundance and nickel tolerance they were further characterized by detailed 16S rRNA gene analysis, DNA-DNA hybridization, fatty acid analysis, heavy metal tolerance, screening for known Ni resistance genes and phenotypic analysis. These strains were found to exhibit different multiple heavy metal resistance characteristics to Ni, Cd, Co, Zn and Cr. On the basis of their physiological and genotypic properties, strains could be grouped with Methylobacterium extorquens and M. mesophilicum. One endophyte, strain iEII3, was found to belong to a novel species for which the name M. goesingense is proposed.

Original language	English
Pages (from-to)	634-644
Journal	Systematic and Applied Microbiology
Volume	29
Issue number	8
DOIs	https://doi.org/10.1016/j.syapm.2006.01.011
State	Published - Dec 2006

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Idris, R., Kuffner, M., Bodrossy, L., Puschenreiter, M., Monchy, S., Wenzel, W., Sessitsch, A., & Mergeay, M. (2006). Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov. Systematic and Applied Microbiology, 29(8), 634-644. https://doi.org/10.1016/j.syapm.2006.01.011

@article{b5a4f088c3bf4096a6b03d062f34ff4c,

title = "Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.",

abstract = "Various pink-pigmented, facultative methylotrophic (PPFM) bacteria (strains iEII3, iEIV1, iEI6, iEII1, iEIII3 iEIII4, iEIII5, iRII1, iRII2, iRIII1, iRIV1 and iRIV2) were obtained from the rhizosphere and endosphere of hyperaccumulating plant Thlaspi goesingense grown in Redschlag, Austria 16. Due to their unexpected diversity, abundance and nickel tolerance they were further characterized by detailed 16S rRNA gene analysis, DNA-DNA hybridization, fatty acid analysis, heavy metal tolerance, screening for known Ni resistance genes and phenotypic analysis. These strains were found to exhibit different multiple heavy metal resistance characteristics to Ni, Cd, Co, Zn and Cr. On the basis of their physiological and genotypic properties, strains could be grouped with Methylobacterium extorquens and M. mesophilicum. One endophyte, strain iEII3, was found to belong to a novel species for which the name M. goesingense is proposed.",

keywords = "16S rRNA gene, heavy metals, DNA-DNA hybridization, rhizosphere, endophytes, Thlaspi goesingense",

author = "Rughia Idris and Melanie Kuffner and Levente Bodrossy and Markus Puschenreiter and S{\'e}bastien Monchy and Walter Wenzel and Angela Sessitsch and Max Mergeay",

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year = "2006",

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doi = "10.1016/j.syapm.2006.01.011",

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Idris, R, Kuffner, M, Bodrossy, L, Puschenreiter, M, Monchy, S, Wenzel, W, Sessitsch, A & Mergeay, M 2006, 'Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.', Systematic and Applied Microbiology, vol. 29, no. 8, pp. 634-644. https://doi.org/10.1016/j.syapm.2006.01.011

TY - JOUR

T1 - Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.

AU - Idris, Rughia

AU - Kuffner, Melanie

AU - Bodrossy, Levente

AU - Puschenreiter, Markus

AU - Monchy, Sébastien

AU - Wenzel, Walter

AU - Sessitsch, Angela

A2 - Mergeay, Max

N1 - Score = 10

PY - 2006/12

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N2 - Various pink-pigmented, facultative methylotrophic (PPFM) bacteria (strains iEII3, iEIV1, iEI6, iEII1, iEIII3 iEIII4, iEIII5, iRII1, iRII2, iRIII1, iRIV1 and iRIV2) were obtained from the rhizosphere and endosphere of hyperaccumulating plant Thlaspi goesingense grown in Redschlag, Austria 16. Due to their unexpected diversity, abundance and nickel tolerance they were further characterized by detailed 16S rRNA gene analysis, DNA-DNA hybridization, fatty acid analysis, heavy metal tolerance, screening for known Ni resistance genes and phenotypic analysis. These strains were found to exhibit different multiple heavy metal resistance characteristics to Ni, Cd, Co, Zn and Cr. On the basis of their physiological and genotypic properties, strains could be grouped with Methylobacterium extorquens and M. mesophilicum. One endophyte, strain iEII3, was found to belong to a novel species for which the name M. goesingense is proposed.

AB - Various pink-pigmented, facultative methylotrophic (PPFM) bacteria (strains iEII3, iEIV1, iEI6, iEII1, iEIII3 iEIII4, iEIII5, iRII1, iRII2, iRIII1, iRIV1 and iRIV2) were obtained from the rhizosphere and endosphere of hyperaccumulating plant Thlaspi goesingense grown in Redschlag, Austria 16. Due to their unexpected diversity, abundance and nickel tolerance they were further characterized by detailed 16S rRNA gene analysis, DNA-DNA hybridization, fatty acid analysis, heavy metal tolerance, screening for known Ni resistance genes and phenotypic analysis. These strains were found to exhibit different multiple heavy metal resistance characteristics to Ni, Cd, Co, Zn and Cr. On the basis of their physiological and genotypic properties, strains could be grouped with Methylobacterium extorquens and M. mesophilicum. One endophyte, strain iEII3, was found to belong to a novel species for which the name M. goesingense is proposed.

KW - 16S rRNA gene

KW - heavy metals

KW - DNA-DNA hybridization

KW - rhizosphere

KW - endophytes

KW - Thlaspi goesingense

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UR - http://knowledgecentre.sckcen.be/so2/bibref/3795

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JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

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ER -