TY - JOUR
T1 - Characterization of the Survival Ability of Cupriavidus metallidurans and Ralstonia pickettii from Space-Related Environments
AU - Mijnendonckx, Kristel
AU - Provoost, Ann
AU - Ott, C.M.
AU - Venkateswaran, Kasthuri
AU - Mahillon, Jacques
AU - Leys, Natalie
AU - Van Houdt, Rob
N1 - Score = 10
PY - 2013/2
Y1 - 2013/2
N2 - Four Cupriavidus metallidurans and eight Ralstonia pickettii isolates from the space industry and the International Space Station (ISS) were characterized in detail. Nine of
the 12 isolates were able to form a biofilm on plastics and all were resistant to several antibiotics. R. pickettii isolates from the surface of the Mars Orbiter prior to flight were 2.5 times more resistant to UV-C254nm radiation compared to the R. pickettii type strain. All isolates showed moderate to high tolerance against at least seven differentmetal ions. They were tolerant to medium to high silver concentrations (0.5–4 μM),
which are higher than the ionic silver disinfectant concentrations measured regularly in the drinking water aboard the ISS. Furthermore, all isolates survived a 23-month exposure to 2 μM AgNO3 in drinking water. These resistance properties are putatively encoded by their endogenous megaplasmids. This study demonstrated that extreme resistance is not required to withstand the disinfection and sterilization procedures
implemented in the ISS and space industry. All isolates acquired moderate to high tolerance against several stressors and can grow in oligotrophic conditions, enabling them to persist in these environments.
AB - Four Cupriavidus metallidurans and eight Ralstonia pickettii isolates from the space industry and the International Space Station (ISS) were characterized in detail. Nine of
the 12 isolates were able to form a biofilm on plastics and all were resistant to several antibiotics. R. pickettii isolates from the surface of the Mars Orbiter prior to flight were 2.5 times more resistant to UV-C254nm radiation compared to the R. pickettii type strain. All isolates showed moderate to high tolerance against at least seven differentmetal ions. They were tolerant to medium to high silver concentrations (0.5–4 μM),
which are higher than the ionic silver disinfectant concentrations measured regularly in the drinking water aboard the ISS. Furthermore, all isolates survived a 23-month exposure to 2 μM AgNO3 in drinking water. These resistance properties are putatively encoded by their endogenous megaplasmids. This study demonstrated that extreme resistance is not required to withstand the disinfection and sterilization procedures
implemented in the ISS and space industry. All isolates acquired moderate to high tolerance against several stressors and can grow in oligotrophic conditions, enabling them to persist in these environments.
KW - cupriavidus metallidurans
KW - ralstonia pickettii
KW - space
KW - resistance
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_127046
UR - http://knowledgecentre.sckcen.be/so2/bibref/9965
U2 - 10.1007/s00248-012-0139-2
DO - 10.1007/s00248-012-0139-2
M3 - Article
SN - 0095-3628
VL - 65
SP - 347
EP - 360
JO - Microbial Ecology
JF - Microbial Ecology
IS - 2
ER -