Chromosome studies in cell cultures of monkeys after application of lead acetate in their drinking water

Chromosome analyses were carried out on lymphocyte cultures (48 h) from 10 monkeys (Cynomolgus monkey) at different time intervals after a strictly controlled lead application. Groups of two monkeys received on each weekday 0, 1.5, 6 and 15 mg of lead acetate in 20 ml of water. Furthermore 2 monkeys were treated with 6 ml of lead acetate but with a calcium free diet. An initial analysis carried after three months, when the monkeys did not yet present signs of lead intoxication, revealed only gaps and fragments but no severe chromosome aberrations. Seven months later, several animals presented signs of lead intoxication, and a few severe aberration configurations could be detected especially in those with a calcium free diet.