Comparable dose estimates of blinded whole blood samples are obtained independently of culture conditions and analytical approaches: Second RENEB gene expression study

Ellina Macaeva, Roel Quintens, Grainne Manning, Matthaeus Majewski, Ralf Kriehuber, Kamil Brzoska, Michael Abend, Sven Doucha-Senf, Dominik Oskamp, Sonja Strunz, Matthias Port, Christophe Badie, Roel Quintens, Sarah Baatout

    Research outputpeer-review


    Purpose: This collaboration of five established European gene expression labs investigated the potential impact of culture conditions on the transcriptional response of peripheral blood to radiation exposure. Materials and methods: Blood from one healthy donor was exposed ex vivo to a Cobalt 60 source to produce a calibration curve in addition to four unknown doses. After exposure, the blood samples were either diluted with RPMI medium or left untouched. After 24-h incubation at 37 C the diluted blood samples were lysed, while the undiluted samples were mixed with the preservative RNALater and all samples were shipped frozen to the participating labs. Samples were processed by each lab using microarray (one lab) and QRT-PCR (four labs). Results: We show that although culture conditions affect the total amount of RNA recovered (p0.5 Gy) measurements (p�.6). Conclusion: This study confirms the robustness of gene expression as a method for biological dosimetry.
    Original languageEnglish
    Pages (from-to)87-98
    JournalInternational Journal of Radiation Biology
    Issue number1
    StatePublished - 14 Sep 2016

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