Detection of mutagenic activity in urine samples using a new concentration procedure

Martine Duverger-van Bogaert; Claudine Stecca; A. Léonard

doi:10.1016/0027-5107(87)90081-9

Detection of mutagenic activity in urine samples using a new concentration procedure

Martine Duverger-van Bogaert
, Claudine Stecca
, A. Léonard

Research output › peer-review

Abstract

A study performed with cyclophosphamide (CP) and nor-nitrogen mustard (NNM), one of its main urinary metabolites, has shown that separation on Polygosil® C-18 resin is preferable to one on XAD-2 resin as a means of concentrating the mutagenic activity present in urine of rats given cytostatics such as CP. Mutagenic activity was detected, using Salmonella typhimurium tester strain TA1535. While NNM is irreversibly bound to XAD-2 resin, it can be recovered after elution with methanol on Polygosil C-18. The better efficacy of Polygosil® C-18 in concentrating CP and its metabolite(s) was confirmed with experiments with urine of rats treated with increasing doses of CP.

Original language	English
Pages (from-to)	21-24
Number of pages	4
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	178
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(87)90081-9
State	Published - May 1987

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/0027-5107(87)90081-9

Cite this

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title = "Detection of mutagenic activity in urine samples using a new concentration procedure",

abstract = "A study performed with cyclophosphamide (CP) and nor-nitrogen mustard (NNM), one of its main urinary metabolites, has shown that separation on Polygosil{\textregistered} C-18 resin is preferable to one on XAD-2 resin as a means of concentrating the mutagenic activity present in urine of rats given cytostatics such as CP. Mutagenic activity was detected, using Salmonella typhimurium tester strain TA1535. While NNM is irreversibly bound to XAD-2 resin, it can be recovered after elution with methanol on Polygosil C-18. The better efficacy of Polygosil{\textregistered} C-18 in concentrating CP and its metabolite(s) was confirmed with experiments with urine of rats treated with increasing doses of CP.",

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AU - Duverger-van Bogaert, Martine

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AU - Léonard, A.

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AB - A study performed with cyclophosphamide (CP) and nor-nitrogen mustard (NNM), one of its main urinary metabolites, has shown that separation on Polygosil® C-18 resin is preferable to one on XAD-2 resin as a means of concentrating the mutagenic activity present in urine of rats given cytostatics such as CP. Mutagenic activity was detected, using Salmonella typhimurium tester strain TA1535. While NNM is irreversibly bound to XAD-2 resin, it can be recovered after elution with methanol on Polygosil C-18. The better efficacy of Polygosil® C-18 in concentrating CP and its metabolite(s) was confirmed with experiments with urine of rats treated with increasing doses of CP.

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KW - Nor-nitrogen mustard

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