@article{4de73c73da864e41a1d8d90d26c64a3a,
title = "Development and implementation of a simulated microgravity setup for edible cyanobacteria",
abstract = "Regenerative life support systems for space crews recycle waste into water, food, and oxygen using different organisms. The European Space Agency{\textquoteright}s MELiSSA program uses the cyanobacterium Limnospira indica PCC8005 for air revitalization and food production. Before space use, components{\textquoteright} compatibility with reduced gravity was tested. This study introduced a ground analog for microgravity experiments with oxygenic cyanobacteria under continuous illumination, using a random positioning machine (RPM) setup. L. indica PCC8005 grew slower under low-shear simulated microgravity, with proteome analysis revealing downregulation of ribosomal proteins, glutamine synthase, and nitrate uptake transporters, and upregulation of gas vesicle, photosystem I and II, and carboxysome proteins. Results suggested inhibition due to high oxygen partial pressure, causing carbon limitation when cultivated in low-shear simulated microgravity. A thicker stagnant fluid boundary layer reducing oxygen release in simulated microgravity was observed. These findings validate this RPM setup for testing the effects of non-terrestrial gravity on photosynthetic microorganisms.",
keywords = "MELiSSA, Limnospira indica PCC 8005, Space, Mass spectrometry, Microbiology",
author = "Gabriele Ellena and Jana Fahrion and Surya Gupta and Claude-Gilles Dussap and Arianna Mazzoli and Natalie Leys and Felice Mastroleo",
note = "Score=10 Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Publisher Copyright: {\textcopyright} The Author(s) 2024.",
year = "2024",
month = dec,
doi = "10.1038/s41526-024-00436-x",
language = "English",
volume = "10",
journal = "npj Microgravity",
issn = "2373-8065",
publisher = "Nature Portfolio",
number = "1",
}