TY - JOUR
T1 - Differential response to acute low dose radiation in primary and immortalized endothelial cells
AU - Rombouts, Charlotte
AU - Aerts, An
AU - Beck, Michaël
AU - De Vos, Winnok
AU - Van Oostveldt, Patrick
AU - Benotmane, Rafi
AU - Baatout, Sarah
N1 - Score = 10
PY - 2013/10
Y1 - 2013/10
N2 - The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and compared.DNA damage and repair, cell cycle progression, apoptosis and cellular morphology in HUVEC and EA.hy926 were evaluated after exposure to low (0.05 - 0.5 Gy) and high doses (2 and 5 Gy) of acute X-rays.Subtle, but significant increases in DNA double strand breaks (DSB) were observed in HUVEC and EA.hy926 30 min after low dose irradiation (0.05 Gy). Compared to high dose irradiation (2 Gy), relatively more DSB/Gy were formed after low dose irradiation. Also, we observed a dose-dependent increase in apoptotic cells, down to 0.5 Gy in HUVEC and 0.1 Gy in EA.hy926 cells. Furthermore, radiation induced significantly more apoptosis in EA.hy926 compared to HUVEC.
We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. Our results point to a non-linear dose-response relationship for DSB formation in endothelial cells. Furthermore, the observed difference in radiation-induced apoptosis points to a higher radiosensitivity of EA.hy926 compared to HUVEC, which should be taken into account when using these cells as models for studying the endothelium radiation response.
AB - The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and compared.DNA damage and repair, cell cycle progression, apoptosis and cellular morphology in HUVEC and EA.hy926 were evaluated after exposure to low (0.05 - 0.5 Gy) and high doses (2 and 5 Gy) of acute X-rays.Subtle, but significant increases in DNA double strand breaks (DSB) were observed in HUVEC and EA.hy926 30 min after low dose irradiation (0.05 Gy). Compared to high dose irradiation (2 Gy), relatively more DSB/Gy were formed after low dose irradiation. Also, we observed a dose-dependent increase in apoptotic cells, down to 0.5 Gy in HUVEC and 0.1 Gy in EA.hy926 cells. Furthermore, radiation induced significantly more apoptosis in EA.hy926 compared to HUVEC.
We demonstrated for the first time that acute low doses of X-rays induce DNA damage and apoptosis in endothelial cells. Our results point to a non-linear dose-response relationship for DSB formation in endothelial cells. Furthermore, the observed difference in radiation-induced apoptosis points to a higher radiosensitivity of EA.hy926 compared to HUVEC, which should be taken into account when using these cells as models for studying the endothelium radiation response.
KW - low dose X-radiation
KW - endothelial cells
KW - DNA damage
KW - cell cycle
KW - apoptosis
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_131756
UR - http://knowledgecentre.sckcen.be/so2/bibref/10687
U2 - 10.3109/09553002.2013.806831
DO - 10.3109/09553002.2013.806831
M3 - Article
SN - 0955-3002
VL - 89
SP - 841
EP - 850
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 10
ER -