R. pickettii strains are prevalent in water and soil. They have been recovered from many different water sources such as distilled water used in hospitals, dental unit water lines, public drinking water supplies, bottled water, ultrapure industrial water systems, and even from drinking water systems of the Mir space station and the Shuttle. R. pickettii has the ability to survive and thrive in oligotrophic conditions probably mediated by its biodegradative abilities, its large metabolic diversity, and its ability to form biofilms, making them more resistant to biocides and consequently more difficult to eradicate. In addition, R. pickettii has been recovered from a wide range of clinical environments and emerged as an opportunistic pathogen that should not be overlooked as a cause of nosocomial infections. The draft genome sequences reported here will help to elucidate how these strains are able to persist in these strictly controlled environments.