TY - JOUR
T1 - Effect of mild acid pH on the functioning of bacterial membranes in Vibrio cholerae
AU - Hommais, Florence
AU - Laurent-Winter, Christine
AU - Labas, Valérie
AU - Krin, Evelyne
AU - Tendeng, Christian
AU - Soutourina, Olga
AU - Danchin, Antoine
AU - Bertin, Philippe
PY - 2002
Y1 - 2002
N2 - In this paper, we initiated the first two-dimensional electrophoresis map of Vibrio cholerae, the aetiological agent of cholera disease. In this pathogen the efficient adaptation to detrimental conditions plays an important role in its survival in both the aquatic reservoir and human intestine. By proteome analysis we investigated the effect of mild acid treatment on the physiology of V. cholerae. More than 50 proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and database searching. Amongst them, pH regulated proteins belong to various functional classes such as intermediary metabolism and bacterial envelope. Several proteins whose accumulation level was decreased in response to acidic pH are known to be involved in the organization and the functioning of membranes, including lipopolysaccharide. Consistent with this, we observed an increased susceptibility to hydrophobic drugs, a loss of motility and a reduction in the ability to form a biofilm in cells grown at pH 6. Our results suggest that V. cholerae is able to sense a moderate decrease in pH and to modify accordingly its structure and physiology.
AB - In this paper, we initiated the first two-dimensional electrophoresis map of Vibrio cholerae, the aetiological agent of cholera disease. In this pathogen the efficient adaptation to detrimental conditions plays an important role in its survival in both the aquatic reservoir and human intestine. By proteome analysis we investigated the effect of mild acid treatment on the physiology of V. cholerae. More than 50 proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and database searching. Amongst them, pH regulated proteins belong to various functional classes such as intermediary metabolism and bacterial envelope. Several proteins whose accumulation level was decreased in response to acidic pH are known to be involved in the organization and the functioning of membranes, including lipopolysaccharide. Consistent with this, we observed an increased susceptibility to hydrophobic drugs, a loss of motility and a reduction in the ability to form a biofilm in cells grown at pH 6. Our results suggest that V. cholerae is able to sense a moderate decrease in pH and to modify accordingly its structure and physiology.
KW - Acidic response
KW - Antibiotic resistance
KW - Bacterial motility
KW - Biofilm
KW - Comparative genomics
KW - Escherichia coli
KW - Two-dimensional gel electrophoresis
KW - Vibrio cholerae
UR - http://www.scopus.com/inward/record.url?scp=0036253799&partnerID=8YFLogxK
U2 - 10.1002/1615-9861(200205)2:5<571::AID-PROT571>3.0.CO;2-G
DO - 10.1002/1615-9861(200205)2:5<571::AID-PROT571>3.0.CO;2-G
M3 - Article
C2 - 11987131
AN - SCOPUS:0036253799
SN - 1615-9853
VL - 2
SP - 571
EP - 579
JO - Proteomics
JF - Proteomics
IS - 5
ER -