TY - JOUR
T1 - Effect of X- and Gamma Rays on DNA in Human Cells
AU - Faraj, Kharman A.
AU - Elias, Mazin M.
AU - Al-Mashhadani, Asia H.
AU - Baatout, Sarah
A2 - Jacquet, Paul
N1 - Score = 10
PY - 2011/5/5
Y1 - 2011/5/5
N2 - The purpose of this study was to investigate the effect of different doses and dose rates of x-rays and Co-60 gamma rays on DNA in normal human mononuclear cells. The cells were irradiated with x-ray doses (0.25, 0.5, 1, 2, 4) Gy at dose rate 0.28 Gy/min or with doses (15.625, 31.25, 62.5, 125, 250) mGy at dose rate 20 mGy/min or with X-ray doses (0.25, 0.5, 1, 2, 4 Gy at dose rate 2Gy/hr. The amount of 8-oxoguanine were detected by fluorescein-isothiocyanate (FITC) labeled antibodies. Using flow cytometry, the number of 8-oxoguanine /cell was calculated by calibration using spheric particles. Significant damage of DNA was observed for both x and rays. We concluded that DNA damage depends on dose and dose rate, and it is good biomarker for radiation response.
AB - The purpose of this study was to investigate the effect of different doses and dose rates of x-rays and Co-60 gamma rays on DNA in normal human mononuclear cells. The cells were irradiated with x-ray doses (0.25, 0.5, 1, 2, 4) Gy at dose rate 0.28 Gy/min or with doses (15.625, 31.25, 62.5, 125, 250) mGy at dose rate 20 mGy/min or with X-ray doses (0.25, 0.5, 1, 2, 4 Gy at dose rate 2Gy/hr. The amount of 8-oxoguanine were detected by fluorescein-isothiocyanate (FITC) labeled antibodies. Using flow cytometry, the number of 8-oxoguanine /cell was calculated by calibration using spheric particles. Significant damage of DNA was observed for both x and rays. We concluded that DNA damage depends on dose and dose rate, and it is good biomarker for radiation response.
KW - X-irradiation
KW - lymphocytes
KW - apoptosis
KW - DNA damage
KW - 8-oxoG
KW - flow cytometry
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_113613
UR - http://knowledgecentre.sckcen.be/so2/bibref/8019
M3 - Article
SN - 1450-216X
VL - 53
SP - 470
EP - 476
JO - European Journal of Scientific Research
JF - European Journal of Scientific Research
IS - 3
ER -