Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts

Paul F. Lurquin

doi:10.1093/nar/6.12.3773

Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts

Paul F. Lurquin

Research output › peer-review

Abstract

Lecithin and lecithin/cholesterol liposomes formed in aqueous solu tions of DNA entrap covalently closed circular, open circular and linear DNA molecules of size up to at least 13 kilobases. The sequestered DNA molecules are efficiently protected against exogenous deoxyribonuclease action although nicking and linearization of circular DNA can be observed. The size of these liposomes ranges from approximately 0.5 to 7.5 μ with an average of 2.5-4 μ. DNA filled liposomes strongly interact with plant protoplasts under conditions inducing protoplast fusion. Results suggest that sequestered plasmid DNA can be transferred to protoplsst nuclei.

Original language	English
Pages (from-to)	3773-3784
Number of pages	12
Journal	Nucleic Acids Research
Volume	6
Issue number	12
DOIs	https://doi.org/10.1093/nar/6.12.3773
State	Published - 24 Aug 1979

Funding

Acknowledgements I gratefully thank G.B. Gerber for the use of the MOP-AMO 3 digitizer, J.M. Nuyten for his help in taking the fluorescence micrographs and A. Liittke for suggesting the use of DAPI. Thanks are also due to L. Ledoux and M. Mergeay for critically reading the manuscript. Supported in part by NATO Research Grant 1447.

Funders	Funder number
Not added	1447

ASJC Scopus subject areas

Genetics

Access to Document

10.1093/nar/6.12.3773

Cite this

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