TY - JOUR
T1 - EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology
AU - Vandevoorde, Charlot
AU - Gomolka, Maria
AU - Roessler, Ute
AU - Samaga, Daniel
AU - Lindholm, Carita
AU - Fernet, Marie
AU - Hall, Janet
AU - Pernot, Eileen
AU - El Saghire, Houssein
AU - Baatout, Sarah
AU - Kesminiene, Ausrele
AU - Thierens, Hubert
A2 - Vanmarcke, Hans
A2 - Quintens, Roel
N1 - Score = 10
PY - 2015/8
Y1 - 2015/8
N2 - Purpose : To conduct a feasibility study on the application of the gamma-H2AX foci assay as an exposure biomarker in a prospective multicentre paediatric radiology setting.
A set of in vitro experiments was performed to evaluate technical hurdles related to biological sample collection in a paediatric radiology setting, processing and storing of blood samples the reliability of foci scoring for low doses, as well as the impact of contrast agent administration as potential confounding factor. Given the exploratory nature of this study and the ethical constraints related to paediatric blood sampling, blood samples from adult volunteers were used for these experiments. In order to test the feasibility of pooling the gamma-H2AX data when different centres are involved in an international multicentre study, two intercomparison studies in the low-dose range (10 – 500 mGy) were performed.
AB - Purpose : To conduct a feasibility study on the application of the gamma-H2AX foci assay as an exposure biomarker in a prospective multicentre paediatric radiology setting.
A set of in vitro experiments was performed to evaluate technical hurdles related to biological sample collection in a paediatric radiology setting, processing and storing of blood samples the reliability of foci scoring for low doses, as well as the impact of contrast agent administration as potential confounding factor. Given the exploratory nature of this study and the ethical constraints related to paediatric blood sampling, blood samples from adult volunteers were used for these experiments. In order to test the feasibility of pooling the gamma-H2AX data when different centres are involved in an international multicentre study, two intercomparison studies in the low-dose range (10 – 500 mGy) were performed.
KW - gamma-H2AX foci
KW - computed tomography
KW - DNA damage
KW - molecular radiobiology
KW - low dose effects
KW - epidemiology
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_140810
UR - http://knowledgecentre.sckcen.be/so2/bibref/13048
U2 - 10.3109/09553002.2015.1047987
DO - 10.3109/09553002.2015.1047987
M3 - Article
VL - 91
SP - 653
EP - 663
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
SN - 0955-3002
IS - 8
ER -