TY - GEN
T1 - ESA IBER-2: Molecular and Cellular Changes in Human Endothelial Cells in Response to Nickel Ion Irradiation (CORALS project)
AU - Moreels, Marjan
AU - Quintens, Roel
AU - De Vos, Winnok
AU - Beck, Michaël
AU - Tabury, Kevin
AU - Suetens, Annelies
AU - Abouelaradat, Khalil
AU - Dieriks, Birger
AU - Ernst, Eric
AU - Lee, Ryonfa
AU - Lambert, Charles
AU - Van Oostveldt, Patrick
AU - Baatout, Sarah
N1 - Score = 3
PY - 2013/2
Y1 - 2013/2
N2 - On Earth, most radiation exposures (medical and natural background) consist of low-linear energy transfer (LET) photons. In space, astronauts are exposed to higher doses and to more varied types of radiation. Cosmic radiation mainly consists of high-energy protons and high-Z and -energy (HZE) particles. These high-LET particles are predicted to account for most of the radiation induced health effects. In this regard, further analysis of the biological effects of HZE particles is essential. In the present study, endothelial cells were irradiated with different doses of nickel ions produced in the synchrotron at GSI (Darmstadt, Germany). After different time points, RNA was extracted for genome-wide analysis and supernatants were collected for multiplex cytokine assay. DNA double strand breaks were detected using γH2AX staining. Our results demonstrated that nickel irradiation induced molecular and cellular changes in human endothelial cells. Further analysis is ongoing to confirm the obtained data and to further explore the biological effects after nickel ion exposure.
AB - On Earth, most radiation exposures (medical and natural background) consist of low-linear energy transfer (LET) photons. In space, astronauts are exposed to higher doses and to more varied types of radiation. Cosmic radiation mainly consists of high-energy protons and high-Z and -energy (HZE) particles. These high-LET particles are predicted to account for most of the radiation induced health effects. In this regard, further analysis of the biological effects of HZE particles is essential. In the present study, endothelial cells were irradiated with different doses of nickel ions produced in the synchrotron at GSI (Darmstadt, Germany). After different time points, RNA was extracted for genome-wide analysis and supernatants were collected for multiplex cytokine assay. DNA double strand breaks were detected using γH2AX staining. Our results demonstrated that nickel irradiation induced molecular and cellular changes in human endothelial cells. Further analysis is ongoing to confirm the obtained data and to further explore the biological effects after nickel ion exposure.
KW - Space radiation
KW - HZE particles
KW - gene expression
KW - DNA damage
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_128597
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_128597_2
UR - http://knowledgecentre.sckcen.be/so2/bibref/10180
M3 - In-proceedings paper
SN - 978-92-9221-270-4
T3 - Spacebooks Online
BT - SP-706 Life in Space for Life on Earth
CY - Noordwijk, Netherlands
T2 - Life in Space for Life on Earth
Y2 - 17 June 2012 through 22 June 2012
ER -