Evidence of a threshold x-ray dose for sensitizing stem-cell spermatogonia of the mouse to the induction of chromosomal translocations by a second larger one

Paul P.W. van Buul; A. Léonard

doi:10.1016/0027-5107(80)90061-5

Evidence of a threshold x-ray dose for sensitizing stem-cell spermatogonia of the mouse to the induction of chromosomal translocations by a second larger one

Paul P.W. van Buul, A. Léonard

Research output › peer-review

Abstract

The effect of different small conditioning doses of X-rays on the production of reciprocal translocations in stem-cell spermatogonia of the mouse (scored in spermatocytes) by a second larger dose have been examined. Fractionation regimes of 25 + 975 R, 50 + 950 R, 75 + 925 R and 100 + 900 R, all with 24 h between the fractions, were applied. The size of the first fraction strongly affected the frequency of induced translocations by the second one, and a kind of threshold dose, somewhere between 75 and 100 R existed for conditioning the spermatogonial population: the translocation yield after 25 + 975 R was 3.3 %, after 50 + 950 R it was 5.0%, and after 75 + 925 R it was 5.1%; whereas 100 + 900 R resulted in 16.1% translocations. It is difficult to explain this observed threshold effect by known biological processes so far held responsible for the conditioning effect.

Original language	English
Pages (from-to)	95-101
Number of pages	7
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(80)90061-5
State	Published - Mar 1980

Funding

Part of the work was supported by the J.A. Cohen Institute, Interuniversity Institute for Radiopathology and Radiation Protection, the Association between Euratom and the University of Leiden, contract No. 052-64-I-BIAN, by Research Contract Euratom/C.E.N. 095-72-I-BIO B and by grants from the "Fonds de la Recherche Scientifique Fondamentale Collective".

Funders	Funder number
Leiden University	052-64-I-BIAN
Horizon Europe

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

Access to Document

10.1016/0027-5107(80)90061-5

Cite this

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title = "Evidence of a threshold x-ray dose for sensitizing stem-cell spermatogonia of the mouse to the induction of chromosomal translocations by a second larger one",

abstract = "The effect of different small conditioning doses of X-rays on the production of reciprocal translocations in stem-cell spermatogonia of the mouse (scored in spermatocytes) by a second larger dose have been examined. Fractionation regimes of 25 + 975 R, 50 + 950 R, 75 + 925 R and 100 + 900 R, all with 24 h between the fractions, were applied. The size of the first fraction strongly affected the frequency of induced translocations by the second one, and a kind of threshold dose, somewhere between 75 and 100 R existed for conditioning the spermatogonial population: the translocation yield after 25 + 975 R was 3.3 %, after 50 + 950 R it was 5.0%, and after 75 + 925 R it was 5.1%; whereas 100 + 900 R resulted in 16.1% translocations. It is difficult to explain this observed threshold effect by known biological processes so far held responsible for the conditioning effect.",

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