Evidence of a threshold x-ray dose for sensitizing stem-cell spermatogonia of the mouse to the induction of chromosomal translocations by a second larger one

Paul P.W. van Buul; A. Léonard

doi:10.1016/0027-5107(80)90061-5

Evidence of a threshold x-ray dose for sensitizing stem-cell spermatogonia of the mouse to the induction of chromosomal translocations by a second larger one

Paul P.W. van Buul, A. Léonard

Research output › peer-review

Abstract

The effect of different small conditioning doses of X-rays on the production of reciprocal translocations in stem-cell spermatogonia of the mouse (scored in spermatocytes) by a second larger dose have been examined. Fractionation regimes of 25 + 975 R, 50 + 950 R, 75 + 925 R and 100 + 900 R, all with 24 h between the fractions, were applied. The size of the first fraction strongly affected the frequency of induced translocations by the second one, and a kind of threshold dose, somewhere between 75 and 100 R existed for conditioning the spermatogonial population: the translocation yield after 25 + 975 R was 3.3 %, after 50 + 950 R it was 5.0%, and after 75 + 925 R it was 5.1%; whereas 100 + 900 R resulted in 16.1% translocations. It is difficult to explain this observed threshold effect by known biological processes so far held responsible for the conditioning effect.

Original language	English
Pages (from-to)	95-101
Number of pages	7
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(80)90061-5
State	Published - Mar 1980

Funding

Funders	Funder number
Leiden University	052-64-I-BIAN
Horizon Europe

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

Access to Document

10.1016/0027-5107(80)90061-5

Cite this

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title = "Evidence of a threshold x-ray dose for sensitizing stem-cell spermatogonia of the mouse to the induction of chromosomal translocations by a second larger one",

abstract = "The effect of different small conditioning doses of X-rays on the production of reciprocal translocations in stem-cell spermatogonia of the mouse (scored in spermatocytes) by a second larger dose have been examined. Fractionation regimes of 25 + 975 R, 50 + 950 R, 75 + 925 R and 100 + 900 R, all with 24 h between the fractions, were applied. The size of the first fraction strongly affected the frequency of induced translocations by the second one, and a kind of threshold dose, somewhere between 75 and 100 R existed for conditioning the spermatogonial population: the translocation yield after 25 + 975 R was 3.3 %, after 50 + 950 R it was 5.0%, and after 75 + 925 R it was 5.1%; whereas 100 + 900 R resulted in 16.1% translocations. It is difficult to explain this observed threshold effect by known biological processes so far held responsible for the conditioning effect.",

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