TY - JOUR
T1 - Exposure to ionizing radiation affects the growth of ectomycorrhizal fungi and induces increased melanin production and increased capacities of reactive oxygen species scavenging enzymes
AU - Kothamasi, David
AU - Wannijn, Jean
AU - Van Hees, May
AU - Nauts, Robin
AU - Van Gompel, Axel
AU - Vanhoudt, Nathalie
AU - Vandenhove, Hildegarde
N1 - Score=10
PY - 2019/1/2
Y1 - 2019/1/2
N2 - Ectomycorrhizal (EM) fungi form symbioses with dominant tree families in boreal, temperate and tropical ecosystems and are important drivers of ecosystem function. EM fungal hyphae extend over a large area making them susceptible to enhanced radiation levels from naturally occurring or anthropogenically originating radioisotopes in the rhizosphere. In this study, the in-vitro effects of ionizing radiation on the growth and biomass of EM fungi Suillus luteus, S. bovinus and Rhizopogon luteolus were investigated. EM fungal cultures were exposed to gamma radiation from a 137Cs source for 137 h in darkness at 21 °C at dose rates of 404, 108.5 and 54.9 mGy h−1 resulting in total absorbed doses of 55.21, 14.82 and 7.50 Gy respectively. Cultures grown in the dark at 21 °C but not exposed to the 137Cs source served as the control. Our results show that EM fungi vary in their sensitivity to ionizing radiation. EM fungi used in this study produced melanin and reactive oxygen species
scavenging enzymes such as catalase and superoxide dismutase as a response to ionizing radiation.
AB - Ectomycorrhizal (EM) fungi form symbioses with dominant tree families in boreal, temperate and tropical ecosystems and are important drivers of ecosystem function. EM fungal hyphae extend over a large area making them susceptible to enhanced radiation levels from naturally occurring or anthropogenically originating radioisotopes in the rhizosphere. In this study, the in-vitro effects of ionizing radiation on the growth and biomass of EM fungi Suillus luteus, S. bovinus and Rhizopogon luteolus were investigated. EM fungal cultures were exposed to gamma radiation from a 137Cs source for 137 h in darkness at 21 °C at dose rates of 404, 108.5 and 54.9 mGy h−1 resulting in total absorbed doses of 55.21, 14.82 and 7.50 Gy respectively. Cultures grown in the dark at 21 °C but not exposed to the 137Cs source served as the control. Our results show that EM fungi vary in their sensitivity to ionizing radiation. EM fungi used in this study produced melanin and reactive oxygen species
scavenging enzymes such as catalase and superoxide dismutase as a response to ionizing radiation.
KW - Ectomycorrhizal fungi
KW - Ionizing radiation
KW - Melanin
KW - Catalase
KW - Superoxide dismutase
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/31357426
U2 - 10.1016/j.jenvrad.2018.11.005
DO - 10.1016/j.jenvrad.2018.11.005
M3 - Article
SN - 0265-931X
VL - 197
SP - 16
EP - 22
JO - Journal of environmental radioactivity
JF - Journal of environmental radioactivity
ER -