Cupriavidus metallidurans H1130 was repeatedly isolated from different blood culture sets taken from a patient suffering from significant nosocomial septicemia. Here, we announce the H1130 genome sequence for use in comparative analyses and for ex-ploring the adaptation and pathogenic potential of this bacterium. C. metallidurans isolates are increasingly being recovered from medically relevant sources, such as patients with cystic fibrosis and human cerebrospinal fluid (e.g., strain CCUG43015, de- posited directly to the Culture Collection, University of Göteborg). Recently, the first case of invasive infection by C. metallidurans was reported (13). Four out of 5 blood culture sets taken over a period of 6 days from a patient with signs of sepsis were positive for a Gram-negative rod that was identified as C. metallidurans (GenBank accession no. GU230889). The genome of this isolate, designated H1130, was sequenced for comparative analyses and for exploring the adaptation and pathogenic potential of this bacterium.