We announce the genome sequence of Serratia plymuthica strain RVH1. Prevalent in soil, air, and water, Serratia species are commonly associated with raw food materials and are often implicated in the spoilage of various foods. In addition, as opportunistic pathogens, they pose a foodborne health hazard and cause nosocomial infections. S. plymuthica RVH1 is being studied for its psychrotolerance mechanisms and its LuxR-LuxI-type quorum-sensing (QS) system based on N-acyl-L-homoserine lactones. The latter controls the production of primary metabolites (switch from mixed acid to acetoin and butanediol fermentation), the polyamino antibiotics zeamine, zeamine I, and zeamine II, and extracellular enzymes. These QS-regulated traits provide a selective advantage in challenging and competitive environments.