Genome-wide transcriptional analysis suggests hydrogenase- and nitrogenase-mediated hydrogen production in Clostridium butyricum CWBI 1009

Magdalena Calusinska, Christopher Hamilton, Pieter Monsieurs, Gregory Mathy, Natalie Leys, Fabrice Franck, Bernard Joris, Philippe Thonart, Serge Hiligsmann, Annick Wilmotte, Rob Van Houdt, Hugo Moors

    Research outputpeer-review


    In this study transcriptomic and proteomic analyses of the hydrogen-producing bacterium Clostridium butyricum CWBI 1009 were carried out to provide a biomolecular overview of the changes that occur when the metabolism shifts to H2 production. The growth, H2-production, and glucose-fermentation profiles were monitored in 20 L batch bioreactors under unregulated-pH and fixed-pH conditions (pH 7.3 and 5.2). Conspicuous differences were observed in the bioreactor performances and cellular metabolisms for all the tested metabolites, and they were pH dependent. During unregulated-pH glucose fermentation increased H2 production was associated with concurrent strong up-regulation of the nitrogenase coding genes. However, no such concurrent up-regulation of the [FeFe] hydrogenase genes was observed. The overall results suggest, for the first time, that environmental factors may determine whether H2 production in C. butyricum CWBI 1009 is mediated by the hydrogenases and/or the nitrogenase.
    Original languageEnglish
    Pages (from-to)1-16
    JournalBiotechnology for Biofuels
    Issue number27
    StatePublished - 22 Feb 2015

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