TY - JOUR
T1 - Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids
AU - Jirka Grosse, Jirka
AU - Wehland, Markus
AU - Pietsch, Jessica
AU - Schulz, Herbert
AU - Saar, Katrin
AU - Hübner, Norbert
AU - Eilles, Christoph
AU - Bauer, Johann
AU - Abouelaradat, Khalil
AU - Baatout, Sarah
AU - Ma, Xiao
AU - Infanger, Manfred
AU - Hemmersbach, Ruth
AU - Grimm, Daniela
A2 - Moreels, Marjan
N1 - Score = 10
PY - 2012/12
Y1 - 2012/12
N2 - This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.
AB - This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.
KW - gene expression
KW - simulated microgravity
KW - cytokines
KW - neoangiogenesis
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_125970
UR - http://knowledgecentre.sckcen.be/so2/bibref/9785
U2 - 10.1096/fj.12-215749
DO - 10.1096/fj.12-215749
M3 - Article
VL - 26
SP - 5124
EP - 5140
JO - The FASEB Journal
JF - The FASEB Journal
SN - 0892-6638
IS - 12
ER -