Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

Jirka Jirka Grosse, Markus Wehland, Jessica Pietsch, Herbert Schulz, Katrin Saar, Norbert Hübner, Christoph Eilles, Johann Bauer, Khalil Abouelaradat, Sarah Baatout, Xiao Ma, Manfred Infanger, Ruth Hemmersbach, Daniela Grimm, Marjan Moreels

    Research outputpeer-review


    This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.
    Original languageEnglish
    Pages (from-to)5124-5140
    JournalThe FASEB Journal
    Issue number12
    StatePublished - Dec 2012

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