Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

Jirka Jirka Grosse; Markus Wehland; Jessica Pietsch; Herbert Schulz; Katrin Saar; Norbert Hübner; Christoph Eilles; Johann Bauer; Khalil Abouelaradat; Sarah Baatout; Xiao Ma; Manfred Infanger; Ruth Hemmersbach; Daniela Grimm; Marjan Moreels

doi:10.1096/fj.12-215749

Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

Jirka Jirka Grosse, Markus Wehland, Jessica Pietsch, Herbert Schulz, Katrin Saar, Norbert Hübner, Christoph Eilles, Johann Bauer, Khalil Abouelaradat, Sarah Baatout, Xiao Ma, Manfred Infanger, Ruth Hemmersbach, Daniela Grimm, Marjan Moreels

Research output › peer-review

Abstract

This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.

Original language	English
Pages (from-to)	5124-5140
Journal	The FASEB Journal
Volume	26
Issue number	12
DOIs	https://doi.org/10.1096/fj.12-215749
State	Published - Dec 2012

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Jirka Grosse, J., Wehland, M., Pietsch, J., Schulz, H., Saar, K., Hübner, N., Eilles, C., Bauer, J., Abouelaradat, K., Baatout, S., Ma, X., Infanger, M., Hemmersbach, R., Grimm, D., & Moreels, M. (2012). Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids. The FASEB Journal, 26(12), 5124-5140. https://doi.org/10.1096/fj.12-215749

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title = "Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids",

abstract = "This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.",

keywords = "gene expression, simulated microgravity, cytokines, neoangiogenesis",

author = "{Jirka Grosse}, Jirka and Markus Wehland and Jessica Pietsch and Herbert Schulz and Katrin Saar and Norbert H{\"u}bner and Christoph Eilles and Johann Bauer and Khalil Abouelaradat and Sarah Baatout and Xiao Ma and Manfred Infanger and Ruth Hemmersbach and Daniela Grimm and Marjan Moreels",

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Jirka Grosse, J, Wehland, M, Pietsch, J, Schulz, H, Saar, K, Hübner, N, Eilles, C, Bauer, J, Abouelaradat, K, Baatout, S, Ma, X, Infanger, M, Hemmersbach, R, Grimm, D & Moreels, M 2012, 'Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids', The FASEB Journal, vol. 26, no. 12, pp. 5124-5140. https://doi.org/10.1096/fj.12-215749

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T1 - Gravity-sensitive signaling drives 3-dimensional formation of multicellular thyroid cancer spheroids

AU - Jirka Grosse, Jirka

AU - Wehland, Markus

AU - Pietsch, Jessica

AU - Schulz, Herbert

AU - Saar, Katrin

AU - Hübner, Norbert

AU - Eilles, Christoph

AU - Bauer, Johann

AU - Abouelaradat, Khalil

AU - Baatout, Sarah

AU - Ma, Xiao

AU - Infanger, Manfred

AU - Hemmersbach, Ruth

AU - Grimm, Daniela

A2 - Moreels, Marjan

N1 - Score = 10

PY - 2012/12

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N2 - This study focused on the effects induced by a random positioning machine (RPM) on FTC-133 thyroid cancer cells and evaluated signaling elements involved in 3-dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1-g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF-_B p65 protein and apoptosis than 1-g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative realtime PCR using the same samples. Thus, we concluded that the signaling elements IL-6, IL-8, OPN, TLN1, and CTGF are involved with NF-_B p65 in RPM-dependent thyroid carcinoma cell spheroid formation.

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KW - gene expression

KW - simulated microgravity

KW - cytokines

KW - neoangiogenesis

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