Growing blood vessels in space: The SPHEROIDS project

Marcus Krüger; Sascha Kopp; Markus Wehland; Johann Bauer; Sarah Baatout; Marjan Moreels; Marcel Egli; Thomas J. Corydon; Manfred Infanger; Daniela Grimm

Growing blood vessels in space: The SPHEROIDS project

Marcus Krüger, Sascha Kopp, Markus Wehland, Johann Bauer, Sarah Baatout, Marjan Moreels, Marcel Egli, Thomas J. Corydon, Manfred Infanger, Daniela Grimm

Radiobiology

Research output › peer-review

Abstract

Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

Original language	English
Title of host publication	69th International astronautical congres - IAC 2018
Publisher	Curran Associates, Inc.
Pages	652-660
Number of pages	9
Volume	1
Edition	2018
ISBN (Print)	9781510881655
State	Published - Apr 2019
Event	AIC 2018 - 69th International astronautical congres - Bremen Duration: 1 Oct 2018 → 5 Oct 2018

Conference

Conference	AIC 2018 - 69th International astronautical congres
Country/Territory	Germany
City	Bremen
Period	2018-10-01 → 2018-10-05

Access to Document

http://www.proceedings.com/47918.htmlLicense: Unspecified

Cite this

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title = "Growing blood vessels in space: The SPHEROIDS project",

abstract = "Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.",

keywords = "Microgravity, Spaceflight, Random positioning machine, 3D growth, Spheroids, Tubular structures",

author = "Marcus Kr{\"u}ger and Sascha Kopp and Markus Wehland and Johann Bauer and Sarah Baatout and Marjan Moreels and Marcel Egli and Corydon, {Thomas J.} and Manfred Infanger and Daniela Grimm",

note = "Score=3; AIC 2018 - 69th International astronautical congres ; Conference date: 01-10-2018 Through 05-10-2018",

year = "2019",

month = apr,

language = "English",

isbn = "9781510881655",

volume = "1",

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booktitle = "69th International astronautical congres - IAC 2018",

publisher = "Curran Associates, Inc.",

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}

Krüger, M, Kopp, S, Wehland, M, Bauer, J, Baatout, S, Moreels, M, Egli, M, Corydon, TJ, Infanger, M & Grimm, D 2019, Growing blood vessels in space: The SPHEROIDS project. in 69th International astronautical congres - IAC 2018. 2018 edn, vol. 1, Curran Associates, Inc., pp. 652-660, AIC 2018 - 69th International astronautical congres, Bremen, Germany, 2018-10-01. <http://www.proceedings.com/47918.html>

TY - GEN

T1 - Growing blood vessels in space

T2 - AIC 2018 - 69th International astronautical congres

AU - Krüger, Marcus

AU - Kopp, Sascha

AU - Wehland, Markus

AU - Bauer, Johann

AU - Baatout, Sarah

AU - Moreels, Marjan

AU - Egli, Marcel

AU - Corydon, Thomas J.

AU - Infanger, Manfred

AU - Grimm, Daniela

N1 - Score=3

PY - 2019/4

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N2 - Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

AB - Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

KW - Microgravity

KW - Spaceflight

KW - Random positioning machine

KW - 3D growth

KW - Spheroids

KW - Tubular structures

UR - https://ecm.sckcen.be/OTCS/llisapi.dll/overview/39624518

M3 - In-proceedings paper

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VL - 1

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EP - 660

BT - 69th International astronautical congres - IAC 2018

PB - Curran Associates, Inc.

Y2 - 1 October 2018 through 5 October 2018

ER -