Haematological disturbances and osmotic shifts in rainbow trout, Oncorhynchus mykiss (Walbaum) under acid and aluminium exposure

H. E. Witters; S. Van Puymbroek; I. Van Den Sande; O. L.J. Vanderborght

doi:10.1007/BF00258984

Haematological disturbances and osmotic shifts in rainbow trout, Oncorhynchus mykiss (Walbaum) under acid and aluminium exposure

H. E. Witters, S. Van Puymbroek, I. Van Den Sande, O. L.J. Vanderborght

Research output › peer-review

Abstract

Elevated values of haematocrit were observed in rainbow trout (Oncorhynchus mykiss) during combined acid and aluminium exposure. Possible causes of this, i.e., decreased plasma volume, swelling of erythrocytes, and/or mobilization of erythrocytes into the blood were investigated. Slight haematocrit increases (10-20%) during mild acid stress (pH 5.0, 25 μmol Ca²⁺·1^-1) were mostly caused by osmotic shifts. Both swelling of erythrocytes rocytes and a significant decrease of the plasma volume were demonstrated in fish at pH 5.0. These osmotic disturbined were greater during acid exposure (pH 5.0) combined with Al (60 μg·1^-1; 200 μg·1^-1). In addition, numbers of erythrocytes increased by 40% compared to acid exposure, which contributed to the severe haematocrit rise (35%) during Al exposure. A contraction of the spleen releasing erythrocytes into the blood is suggested to occur as an adrenergic response to hypoxia, which is observed in fish acutely exposed to Al.

Original language	English
Pages (from-to)	563-571
Number of pages	9
Journal	Journal of Comparative Physiology B
Volume	160
Issue number	5
DOIs	https://doi.org/10.1007/BF00258984
State	Published - Dec 1990

ASJC Scopus subject areas

Biochemistry
Physiology
Ecology, Evolution, Behavior and Systematics
Animal Science and Zoology
Endocrinology

Access to Document

10.1007/BF00258984

Cite this

@article{bddf5082376c4a359d7eddebfffe56cc,

title = "Haematological disturbances and osmotic shifts in rainbow trout, Oncorhynchus mykiss (Walbaum) under acid and aluminium exposure",

abstract = "Elevated values of haematocrit were observed in rainbow trout (Oncorhynchus mykiss) during combined acid and aluminium exposure. Possible causes of this, i.e., decreased plasma volume, swelling of erythrocytes, and/or mobilization of erythrocytes into the blood were investigated. Slight haematocrit increases (10-20%) during mild acid stress (pH 5.0, 25 μmol Ca2+·1-1) were mostly caused by osmotic shifts. Both swelling of erythrocytes rocytes and a significant decrease of the plasma volume were demonstrated in fish at pH 5.0. These osmotic disturbined were greater during acid exposure (pH 5.0) combined with Al (60 μg·1-1; 200 μg·1-1). In addition, numbers of erythrocytes increased by 40% compared to acid exposure, which contributed to the severe haematocrit rise (35%) during Al exposure. A contraction of the spleen releasing erythrocytes into the blood is suggested to occur as an adrenergic response to hypoxia, which is observed in fish acutely exposed to Al.",

keywords = "Aluminium, Haematology, Low pH, Osmotic changes, Rainbow trout",

author = "Witters, {H. E.} and {Van Puymbroek}, S. and {Van Den Sande}, I. and Vanderborght, {O. L.J.}",

year = "1990",

month = dec,

doi = "10.1007/BF00258984",

language = "English",

volume = "160",

pages = "563--571",

journal = "Journal of Comparative Physiology B",

issn = "0174-1578",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "5",

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T1 - Haematological disturbances and osmotic shifts in rainbow trout, Oncorhynchus mykiss (Walbaum) under acid and aluminium exposure

AU - Witters, H. E.

AU - Van Puymbroek, S.

AU - Van Den Sande, I.

AU - Vanderborght, O. L.J.

PY - 1990/12

Y1 - 1990/12

N2 - Elevated values of haematocrit were observed in rainbow trout (Oncorhynchus mykiss) during combined acid and aluminium exposure. Possible causes of this, i.e., decreased plasma volume, swelling of erythrocytes, and/or mobilization of erythrocytes into the blood were investigated. Slight haematocrit increases (10-20%) during mild acid stress (pH 5.0, 25 μmol Ca2+·1-1) were mostly caused by osmotic shifts. Both swelling of erythrocytes rocytes and a significant decrease of the plasma volume were demonstrated in fish at pH 5.0. These osmotic disturbined were greater during acid exposure (pH 5.0) combined with Al (60 μg·1-1; 200 μg·1-1). In addition, numbers of erythrocytes increased by 40% compared to acid exposure, which contributed to the severe haematocrit rise (35%) during Al exposure. A contraction of the spleen releasing erythrocytes into the blood is suggested to occur as an adrenergic response to hypoxia, which is observed in fish acutely exposed to Al.

AB - Elevated values of haematocrit were observed in rainbow trout (Oncorhynchus mykiss) during combined acid and aluminium exposure. Possible causes of this, i.e., decreased plasma volume, swelling of erythrocytes, and/or mobilization of erythrocytes into the blood were investigated. Slight haematocrit increases (10-20%) during mild acid stress (pH 5.0, 25 μmol Ca2+·1-1) were mostly caused by osmotic shifts. Both swelling of erythrocytes rocytes and a significant decrease of the plasma volume were demonstrated in fish at pH 5.0. These osmotic disturbined were greater during acid exposure (pH 5.0) combined with Al (60 μg·1-1; 200 μg·1-1). In addition, numbers of erythrocytes increased by 40% compared to acid exposure, which contributed to the severe haematocrit rise (35%) during Al exposure. A contraction of the spleen releasing erythrocytes into the blood is suggested to occur as an adrenergic response to hypoxia, which is observed in fish acutely exposed to Al.

KW - Aluminium

KW - Haematology

KW - Low pH

KW - Osmotic changes

KW - Rainbow trout

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