TY - JOUR
T1 - Increased radiation sensitivity of an eosinophilic cell line following treatment with epigallocatechin-gallate, resveratrol and curcuma
AU - Baatout, Sarah
AU - Derradji, Hanane
AU - Jacquet, Paul
AU - Mergeay, Max
N1 - Score = 10
PY - 2005/1/15
Y1 - 2005/1/15
N2 - Ionizing radiation is widely used in radiotherapy, in order to promote an apoptotic response in cancerous cells. Since the need of finding new substances that would enhance the radiation-induced apoptosis in cancerous cells is great, we studied the effect of epigallocatechin-gallate (EGCG, a tea component), resveratrol (a wine component) and curcuma on cell proliferation and radiation-induced apoptosis in the human leukaemic cell line, EOL-1, derived from a patient with eosinophilic leukaemia.
Cells were X-irradiated with 0, 2, 4, 6 or 8 Gy and cultured in the presence of EGCG, resveratrol or curcuma (concentrations ranging from 0 to 200 µM) for 1, 2 or 3 days of culture. Cell proliferation was measured using trypan blue exclusion. Apoptosis was evaluated using light microscopy (morphology study after May-Grünwald Giemsa staining) and flow cytometry (annexin-V staining).
Irradiation alone induced a dose-related reduction in cell proliferation and the appearance of polyploid cells in EOL-1 cells. Additionally, EOL-1 cells underwent a dose-related increase of apoptosis which, from the second day on, was accompanied by a dose-related increase of necrosis.
When cells were exposed to EGCG, resveratrol or curcuma alone, a decrease in cell proliferation was observed, beginning from 25 µM EGCG and 50 µM resveratrol and curcuma , while an increase in the percentage of apoptotic cells was noticed from 50 µM EGCG, 100 µM resveratrol and curcuma in EOL-1 cells, after only one day of culture. Simultaneous exposure to X-irradiation and, EGCG, resveratrol or curcuma resulted in a synergistic decrease of cell proliferation as well as in a synergistic increase of apoptosis and necrosis.
AB - Ionizing radiation is widely used in radiotherapy, in order to promote an apoptotic response in cancerous cells. Since the need of finding new substances that would enhance the radiation-induced apoptosis in cancerous cells is great, we studied the effect of epigallocatechin-gallate (EGCG, a tea component), resveratrol (a wine component) and curcuma on cell proliferation and radiation-induced apoptosis in the human leukaemic cell line, EOL-1, derived from a patient with eosinophilic leukaemia.
Cells were X-irradiated with 0, 2, 4, 6 or 8 Gy and cultured in the presence of EGCG, resveratrol or curcuma (concentrations ranging from 0 to 200 µM) for 1, 2 or 3 days of culture. Cell proliferation was measured using trypan blue exclusion. Apoptosis was evaluated using light microscopy (morphology study after May-Grünwald Giemsa staining) and flow cytometry (annexin-V staining).
Irradiation alone induced a dose-related reduction in cell proliferation and the appearance of polyploid cells in EOL-1 cells. Additionally, EOL-1 cells underwent a dose-related increase of apoptosis which, from the second day on, was accompanied by a dose-related increase of necrosis.
When cells were exposed to EGCG, resveratrol or curcuma alone, a decrease in cell proliferation was observed, beginning from 25 µM EGCG and 50 µM resveratrol and curcuma , while an increase in the percentage of apoptotic cells was noticed from 50 µM EGCG, 100 µM resveratrol and curcuma in EOL-1 cells, after only one day of culture. Simultaneous exposure to X-irradiation and, EGCG, resveratrol or curcuma resulted in a synergistic decrease of cell proliferation as well as in a synergistic increase of apoptosis and necrosis.
KW - radiotherapy
KW - cancer
KW - leukemia
KW - resveratrol
KW - tea
KW - curcuma
KW - EGCG
KW - irradiation
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_27301
UR - http://knowledgecentre.sckcen.be/so2/bibref/3258
M3 - Article
SN - 1107-3756
VL - 15
SP - 337
EP - 352
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
ER -