Integrity of glycoprotein complex sugars is required for homing but not for several other membrane‐mediated functions

Robert J. Hooghe; Frank Vander Plaetse; Louis de Saint‐Georges; Michel Janowski; Nicole Schaaf‐Lafontaine; Elisabeth L. Hooghe‐Peters; Peter Dorling

doi:10.1002/cbf.290020209

Integrity of glycoprotein complex sugars is required for homing but not for several other membrane‐mediated functions

Robert J. Hooghe, Frank Vander Plaetse, Louis de Saint‐Georges, Michel Janowski, Nicole Schaaf‐Lafontaine, Elisabeth L. Hooghe‐Peters, Peter Dorling

Research output › peer-review

Abstract

In order to correlate the biochemistry of cell surface carbohydrates with cell function, we have treated cells with swainsonine and followed the biochemical and functional modifications induced by this compound. After treatment with swainsonine, surface glycoproteins had a lower apparent molecular weight and a higher isoelectric point. This is compatible with the replacement of complex carbohydrates by hybrid or high‐mannose carbohydrates. Several functional tests were unaffected. However, swainsonine‐treated cells displayed an altered pattern of in vivo homing, suggesting that carbohydrates play a role in this process.

Original language	English
Pages (from-to)	102-106
Number of pages	5
Journal	Cell biochemistry and function
Volume	2
Issue number	2
DOIs	https://doi.org/10.1002/cbf.290020209
State	Published - Apr 1984

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Cell Biology

Access to Document

10.1002/cbf.290020209

Cite this

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title = "Integrity of glycoprotein complex sugars is required for homing but not for several other membrane‐mediated functions",

abstract = "In order to correlate the biochemistry of cell surface carbohydrates with cell function, we have treated cells with swainsonine and followed the biochemical and functional modifications induced by this compound. After treatment with swainsonine, surface glycoproteins had a lower apparent molecular weight and a higher isoelectric point. This is compatible with the replacement of complex carbohydrates by hybrid or high‐mannose carbohydrates. Several functional tests were unaffected. However, swainsonine‐treated cells displayed an altered pattern of in vivo homing, suggesting that carbohydrates play a role in this process.",

keywords = "Carbohydrates, cytotoxicity, glycosylation, lymphoma, membrane, metastasis, retrovirus",

author = "Hooghe, {Robert J.} and Plaetse, {Frank Vander} and {de Saint‐Georges}, Louis and Michel Janowski and Nicole Schaaf‐Lafontaine and Hooghe‐Peters, {Elisabeth L.} and Peter Dorling",

year = "1984",

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T1 - Integrity of glycoprotein complex sugars is required for homing but not for several other membrane‐mediated functions

AU - Hooghe, Robert J.

AU - Plaetse, Frank Vander

AU - de Saint‐Georges, Louis

AU - Janowski, Michel

AU - Schaaf‐Lafontaine, Nicole

AU - Hooghe‐Peters, Elisabeth L.

AU - Dorling, Peter

PY - 1984/4

Y1 - 1984/4

N2 - In order to correlate the biochemistry of cell surface carbohydrates with cell function, we have treated cells with swainsonine and followed the biochemical and functional modifications induced by this compound. After treatment with swainsonine, surface glycoproteins had a lower apparent molecular weight and a higher isoelectric point. This is compatible with the replacement of complex carbohydrates by hybrid or high‐mannose carbohydrates. Several functional tests were unaffected. However, swainsonine‐treated cells displayed an altered pattern of in vivo homing, suggesting that carbohydrates play a role in this process.

AB - In order to correlate the biochemistry of cell surface carbohydrates with cell function, we have treated cells with swainsonine and followed the biochemical and functional modifications induced by this compound. After treatment with swainsonine, surface glycoproteins had a lower apparent molecular weight and a higher isoelectric point. This is compatible with the replacement of complex carbohydrates by hybrid or high‐mannose carbohydrates. Several functional tests were unaffected. However, swainsonine‐treated cells displayed an altered pattern of in vivo homing, suggesting that carbohydrates play a role in this process.

KW - Carbohydrates

KW - cytotoxicity

KW - glycosylation

KW - lymphoma

KW - membrane

KW - metastasis

KW - retrovirus

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AN - SCOPUS:0021341147

SN - 0263-6484

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