Introduction to the principles and methods underlying the recovery of metagenome‐assembled genomes from metagenomic data

    Research outputpeer-review


    The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it represents a relatively new discipline with various levels of complexity and demands on bioinformatics. The underlying principles and methods used in metagenomics are often seen as common knowledge and often not detailed or fragmented. Therefore, we reviewed these to guide microbiologists in taking the first steps into metagenomics. We specifically focus on a workflow aimed at reconstructing individual genomes, that is, metagenome‐assembled genomes, integrating DNA sequencing, assembly, binning, identification and annotation
    Original languageEnglish
    Article numbere1298
    Pages (from-to)1-19
    Number of pages19
    JournalMicrobiology Open
    Issue number3
    StatePublished - 9 Jun 2022

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