@inbook{24e0ed902a8f45d483c4a829918cb54d,
title = "Investigating in situ natural genetic transformation of Acinetobacter sp. BD413 in biofilms with confocal laser scanning microscopy",
abstract = "In situ monitoring tools are the method of choice to investigate natural genetic transformation of Acinetobacter sp. BD413 in biofilms. The combination of genes encoding for the green fluorescent protein (gfp) or its derivatives as transforming DNA and confocal laser scanning microscopy (CLSM) as an analysis tool can provide for a convenient experimental set-up. The tasks that are necessary to perform an in situ natural genetic transformation study are: construction of the tools for in vivo labeling of transconjugants; control experiments to reduce false positive/negative signals; and design of the mathematical formulae to describe the natural transformation events inside the biofilm.",
keywords = "Confocal Laser Scanning Microscopy (CLSM), Acinetobacter sp. BD413, Natural genetic transformation, Biofilm, In situ quantification, Green fluorescent protein",
author = "Larissa Hendrickx and Stefan Wuertz",
year = "2004",
doi = "10.1007/978-0-306-48573-2_9",
language = "English",
volume = "26",
series = "Genetic engineering",
publisher = "Kluwer Academic Publishers",
pages = "159--173",
booktitle = "Genetic Engineering",
address = "Netherlands",
}