TY - JOUR
T1 - Life-cycle chronic gamma exposure of Arabidopsis Thaliana induces growth effects but no discernable effects on oxidative stress pathways
AU - Vandenhove, Hildegarde
AU - Vanhoudt, Nathalie
AU - Cuypers, Ann
AU - Van Hees, May
AU - Wannijn, Jean
AU - Horemans, Nele
A2 - Vanmarcke, Hans
N1 - Score = 10
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Arabidopsis thaliana was exposed to low-dose chronic gamma irradiation during a full life cycle and several biological responses were investigated. Following 24 days (inflorescence emergence), 34 days (w50% of flowers open) and 54 days
(silice ripening) exposure, plants were harvested and monitored for biometric parameters, capacities of enzymes involved in the antioxidative defence mechanisms (SOD, APOD, GLUR, GPOD, SPOD, CAT, ME), glutathione and ascorbate pool, lipid peroxidation products, altered gene expression of selected genes encoding for antioxidative enzymes or reactive oxygen species production, and DNA integrity. Root fresh weight was significantly reduced after gamma exposure compared to the control at all stages monitored but no significant differences in root weight for the different dose rates applied was observed. Leaf and stem fresh weight were significantly reduced at the highest irradiation level after 54 days exposure only. Also total plant fresh was significantly lower at silice riping and this for the highest and medium dose rate applied. Germination of seeds from the gamma irradiated plants was not hampered. For several of the antioxidative defence enzymes studied, the enzyme capacity was generally stimulated towards flowering but generally no significant effect of dose rate on enzyme capacity was observed.
AB - Arabidopsis thaliana was exposed to low-dose chronic gamma irradiation during a full life cycle and several biological responses were investigated. Following 24 days (inflorescence emergence), 34 days (w50% of flowers open) and 54 days
(silice ripening) exposure, plants were harvested and monitored for biometric parameters, capacities of enzymes involved in the antioxidative defence mechanisms (SOD, APOD, GLUR, GPOD, SPOD, CAT, ME), glutathione and ascorbate pool, lipid peroxidation products, altered gene expression of selected genes encoding for antioxidative enzymes or reactive oxygen species production, and DNA integrity. Root fresh weight was significantly reduced after gamma exposure compared to the control at all stages monitored but no significant differences in root weight for the different dose rates applied was observed. Leaf and stem fresh weight were significantly reduced at the highest irradiation level after 54 days exposure only. Also total plant fresh was significantly lower at silice riping and this for the highest and medium dose rate applied. Germination of seeds from the gamma irradiated plants was not hampered. For several of the antioxidative defence enzymes studied, the enzyme capacity was generally stimulated towards flowering but generally no significant effect of dose rate on enzyme capacity was observed.
KW - Life-cycle
KW - chronic
KW - gamma exposure
KW - Arabidopsis Thaliana
KW - growth effects
KW - discernable effects
KW - oxidative stress pathways
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_107291
UR - http://knowledgecentre.sckcen.be/so2/bibref/7152
U2 - 10.1016/j.plaphy.2010.06.006
DO - 10.1016/j.plaphy.2010.06.006
M3 - Article
SN - 0981-9428
VL - 48
SP - 778
EP - 786
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
IS - 9
ER -