TY - JOUR
T1 - Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells
AU - Abouelaradat, Khalil
AU - Monsieurs, Pieter
AU - Anastasov, Natasa
AU - Atkinson, Mike
AU - Derradji, Hanane
AU - De Meyer, Tim
AU - Bekaert, Sofie
AU - Van Criekinge, Wim
AU - Baatout, Sarah
N1 - Score = 10
PY - 2012/3
Y1 - 2012/3
N2 - The high doses of radiation received in the wake of the Chernobyl incident have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids.
AB - The high doses of radiation received in the wake of the Chernobyl incident have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids.
KW - radiation
KW - thyroid
KW - epigenetics
KW - methylomics
KW - Chernobyl
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_118605
UR - http://knowledgecentre.sckcen.be/so2/bibref/8888
U2 - 10.1016/j.mrfmmm.2011.10.006
DO - 10.1016/j.mrfmmm.2011.10.006
M3 - Article
SN - 0027-5107
VL - 731
SP - 27
EP - 40
JO - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1-2
ER -