TY - JOUR
T1 - Low-dose radiations derived from cone-beam CT induce transient DNA damage and persistent inflammatory reactions in stem cells from deciduous teeth
AU - Piroska, Virag
AU - Hedisu, Mihaela
AU - Soritau, Olga
AU - Perde-Schrepler, Mari
AU - Brie, Ioana
AU - Pall, Emoke
AU - Fischer-Fodor, Eva
AU - Bogdan, Loredana
AU - Lucaciu, Ondine
AU - Belmans, Niels
AU - Moreels, Marjan
AU - Salmon, Benjamin
AU - Jacobs, Reinhilde
N1 - Score=10
PY - 2018/7/2
Y1 - 2018/7/2
N2 - Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive
substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT.
Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4–107.7 mGy, corresponding to 0.5–8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1α, IL-6, IL-8, TNFα in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure.
Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p <0.001 for 3, 5, 8 skull exposures and p <0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p <0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time.
Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population.
AB - Objectives: Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive
substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT.
Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4–107.7 mGy, corresponding to 0.5–8 consecutive skull exposures, respectively. H2AX and MRE11 were detected in whole cells, while IL-1α, IL-6, IL-8, TNFα in supernatants, using enzyme-linked immunosorbent assay (ELISA) at different time points after exposure.
Results: The phosphorylation level of H2AX in DPSCs increased considerably at 0.5 h after exposure (p <0.001 for 3, 5, 8 skull exposures and p <0.05 for 1 skull exposure, respectively). MRE11 response could only be detected for the highest IR dose (p <0.001) in the same interval. CKs secretion increased upon CBCT exposure according to doses and time.
Conclusions: The DPSCs exposure to CBCT induces transient DNA damage and persistent inflammatory reaction in DPSCs drawing the attention on the potential risks of IR exposures and on the importance of dose monitoring in pediatric population.
KW - Cone beam CT
KW - DNA damage
KW - dental stem cells
UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/31022880
U2 - 10.1259/dmfr.20170462
DO - 10.1259/dmfr.20170462
M3 - Article
SN - 0250-832X
VL - 47
JO - Dentomaxillofacial Radiology
JF - Dentomaxillofacial Radiology
M1 - 20170462
ER -