Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability

Irwin Cassells; Maarten Ooms; Andrew Burgoyne; Jozef Cornelis; Stephen Ahenkorah; Michiel Van de Voorde; Thomas Cardinaels; Christophe Deroose; Guy Bormans; Frederik Cleeren

doi:10.1007/s00259-020-04988-4

Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability

Irwin Cassells, Maarten Ooms, Andrew Burgoyne, Jozef Cornelis, Stephen Ahenkorah, Michiel Van de Voorde, Thomas Cardinaels, Christophe Deroose, Guy Bormans, Frederik Cleeren

Radiochemistry

Research output › peer-review

Abstract

Targeted radionuclide therapy using Terbium-161 (161Tb) is a promising approach for β- and Auger electron therapy. Heat-sensitive biomolecules (e.g. nanobodies) are increasingly being used as carriers in radiometal-based pharmaceuticals, however current radiolabelling techniques require heating (>85 °C) incompatible with heat-sensitive carrier molecules. In this study, we evaluated bifunctional chelators that allow 61Tblabelling under mild conditions using human serum albumin (HSA) and assessed the in vivo stability of the constructs.

Original language	English
Title of host publication	European Journal of Nuclear Medicine and Molecular Imaging
Pages	S753
Number of pages	1
DOIs	https://doi.org/10.1007/s00259-020-04988-4
State	Published - 30 Oct 2020
Event	2020 - EANM: European Association of Nuclear Medicine - Online Duration: 22 Oct 2020 → 30 Oct 2020 https://eanm20.eanm.org/

Publication series

Name	European Journal of Nuclear Medicine and Molecular Imaging
Publisher	Springer
Number	Supplement 1
Volume	47
ISSN (Print)	1619-7070

Conference

Conference	2020 - EANM
Period	2020-10-22 → 2020-10-30
Internet address	https://eanm20.eanm.org/

Access to Document

10.1007/s00259-020-04988-4License: Unspecified

Cite this

Cassells, I., Ooms, M., Burgoyne, A., Cornelis, J., Ahenkorah, S., Van de Voorde, M., Cardinaels, T., Deroose, C., Bormans, G., & Cleeren, F. (2020). Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability. In European Journal of Nuclear Medicine and Molecular Imaging (pp. S753). [OP-151] (European Journal of Nuclear Medicine and Molecular Imaging; Vol. 47, No. Supplement 1). https://doi.org/10.1007/s00259-020-04988-4

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abstract = "Targeted radionuclide therapy using Terbium-161 (161Tb) is a promising approach for β- and Auger electron therapy. Heat-sensitive biomolecules (e.g. nanobodies) are increasingly being used as carriers in radiometal-based pharmaceuticals, however current radiolabelling techniques require heating (>85 °C) incompatible with heat-sensitive carrier molecules. In this study, we evaluated bifunctional chelators that allow 61Tblabelling under mild conditions using human serum albumin (HSA) and assessed the in vivo stability of the constructs.",

keywords = "Radiolabelling, Terbium-161, Biodistribution, In vivo stability",

author = "Irwin Cassells and Maarten Ooms and Andrew Burgoyne and Jozef Cornelis and Stephen Ahenkorah and {Van de Voorde}, Michiel and Thomas Cardinaels and Christophe Deroose and Guy Bormans and Frederik Cleeren",

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Cassells, I, Ooms, M, Burgoyne, A, Cornelis, J, Ahenkorah, S, Van de Voorde, M , Cardinaels, T, Deroose, C, Bormans, G & Cleeren, F 2020, Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability. in European Journal of Nuclear Medicine and Molecular Imaging., OP-151, European Journal of Nuclear Medicine and Molecular Imaging, no. Supplement 1, vol. 47, pp. S753, 2020 - EANM, 2020-10-22. https://doi.org/10.1007/s00259-020-04988-4

Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability. / Cassells, Irwin; Ooms, Maarten; Burgoyne, Andrew et al.
European Journal of Nuclear Medicine and Molecular Imaging. 2020. p. S753 OP-151 (European Journal of Nuclear Medicine and Molecular Imaging; Vol. 47, No. Supplement 1).

Research output › peer-review

TY - GEN

T1 - Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability

AU - Cassells, Irwin

AU - Ooms, Maarten

AU - Burgoyne, Andrew

AU - Cornelis, Jozef

AU - Ahenkorah, Stephen

AU - Van de Voorde, Michiel

AU - Cardinaels, Thomas

AU - Deroose, Christophe

AU - Bormans, Guy

AU - Cleeren, Frederik

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AB - Targeted radionuclide therapy using Terbium-161 (161Tb) is a promising approach for β- and Auger electron therapy. Heat-sensitive biomolecules (e.g. nanobodies) are increasingly being used as carriers in radiometal-based pharmaceuticals, however current radiolabelling techniques require heating (>85 °C) incompatible with heat-sensitive carrier molecules. In this study, we evaluated bifunctional chelators that allow 61Tblabelling under mild conditions using human serum albumin (HSA) and assessed the in vivo stability of the constructs.

KW - Radiolabelling

KW - Terbium-161

KW - Biodistribution

KW - In vivo stability

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Cassells I, Ooms M, Burgoyne A, Cornelis J, Ahenkorah S, Van de Voorde M et al. Low temperature radiolabelling of human serum albumin constructs with terbium-161 and ex vivo biodistribution to assess in vivo stability. In European Journal of Nuclear Medicine and Molecular Imaging. 2020. p. S753. OP-151. (European Journal of Nuclear Medicine and Molecular Imaging; Supplement 1). doi: 10.1007/s00259-020-04988-4