Méthode simple pour le dosage quantitatif de l'acide β-amino-isobutyrique dans l'urine humaine par chromatographie sur papier

W. Schietecatte, G. Maes, M. H. Faes, R. Bernardelli

    Research outputpeer-review

    Abstract

    The methods proposed as yet for the dosage of β-aminoisobutyric acid (BAIBA) in urine are tedious, not very sensitive and semi-quantitative. We have elaborated a rather simple method which permits us to measure quantitatively small amounts of BAIBA without need of preliminary demineralization and concentration. Approximately 50 μl of urine are directly applied to paper. After two consecutive developments in the same solvent, the spot of BAIBA is sharply isolated. The coloration is measured by transmission in the densitometer. A doubling of the normal excretion rate of BAIBA can be detected with sufficient precision.

    Translated title of the contributionA simple method for the quantitative determination of β-Aminobutyric Acid in Human Urine by Paper Chromatography
    Original languageFrench
    Pages (from-to)259-262
    Number of pages4
    JournalClinica Chimica Acta
    Volume11
    Issue number3
    DOIs
    StatePublished - Mar 1965

    ASJC Scopus subject areas

    • Biochemistry
    • Clinical Biochemistry
    • Biochemistry, medical

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