Méthode simple pour le dosage quantitatif de l'acide β-amino-isobutyrique dans l'urine humaine par chromatographie sur papier

W. Schietecatte; G. Maes; M. H. Faes; R. Bernardelli

doi:10.1016/0009-8981(65)90071-9

Méthode simple pour le dosage quantitatif de l'acide β-amino-isobutyrique dans l'urine humaine par chromatographie sur papier

W. Schietecatte, G. Maes, M. H. Faes, R. Bernardelli

Research output › peer-review

Abstract

The methods proposed as yet for the dosage of β-aminoisobutyric acid (BAIBA) in urine are tedious, not very sensitive and semi-quantitative. We have elaborated a rather simple method which permits us to measure quantitatively small amounts of BAIBA without need of preliminary demineralization and concentration. Approximately 50 μl of urine are directly applied to paper. After two consecutive developments in the same solvent, the spot of BAIBA is sharply isolated. The coloration is measured by transmission in the densitometer. A doubling of the normal excretion rate of BAIBA can be detected with sufficient precision.

Translated title of the contribution	A simple method for the quantitative determination of β-Aminobutyric Acid in Human Urine by Paper Chromatography
Original language	French
Pages (from-to)	259-262
Number of pages	4
Journal	Clinica Chimica Acta
Volume	11
Issue number	3
DOIs	https://doi.org/10.1016/0009-8981(65)90071-9
State	Published - Mar 1965

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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10.1016/0009-8981(65)90071-9

Cite this

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title = "M{\'e}thode simple pour le dosage quantitatif de l'acide β-amino-isobutyrique dans l'urine humaine par chromatographie sur papier",

abstract = "The methods proposed as yet for the dosage of β-aminoisobutyric acid (BAIBA) in urine are tedious, not very sensitive and semi-quantitative. We have elaborated a rather simple method which permits us to measure quantitatively small amounts of BAIBA without need of preliminary demineralization and concentration. Approximately 50 μl of urine are directly applied to paper. After two consecutive developments in the same solvent, the spot of BAIBA is sharply isolated. The coloration is measured by transmission in the densitometer. A doubling of the normal excretion rate of BAIBA can be detected with sufficient precision.",

author = "W. Schietecatte and G. Maes and Faes, {M. H.} and R. Bernardelli",

year = "1965",

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doi = "10.1016/0009-8981(65)90071-9",

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AU - Schietecatte, W.

AU - Maes, G.

AU - Faes, M. H.

AU - Bernardelli, R.

PY - 1965/3

Y1 - 1965/3

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AB - The methods proposed as yet for the dosage of β-aminoisobutyric acid (BAIBA) in urine are tedious, not very sensitive and semi-quantitative. We have elaborated a rather simple method which permits us to measure quantitatively small amounts of BAIBA without need of preliminary demineralization and concentration. Approximately 50 μl of urine are directly applied to paper. After two consecutive developments in the same solvent, the spot of BAIBA is sharply isolated. The coloration is measured by transmission in the densitometer. A doubling of the normal excretion rate of BAIBA can be detected with sufficient precision.

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