Abstract
The methods proposed as yet for the dosage of β-aminoisobutyric acid (BAIBA)
in urine are tedious, not very sensitive and semi-quantitative. We have elaborated a rather simple method which permits us to measure quantitatively small amounts of BAIBA without need of preliminary demineralization and concentration. Approximately 50 /A of urine are directly applied to paper. After two consecutive developments in the same solvent, the spot of BAIBA is sharply isolated. The coloration is measured by transmission in the densitometer. A doubling of the normal excretion rate of BAIBA can be detected with sufficient precision.
in urine are tedious, not very sensitive and semi-quantitative. We have elaborated a rather simple method which permits us to measure quantitatively small amounts of BAIBA without need of preliminary demineralization and concentration. Approximately 50 /A of urine are directly applied to paper. After two consecutive developments in the same solvent, the spot of BAIBA is sharply isolated. The coloration is measured by transmission in the densitometer. A doubling of the normal excretion rate of BAIBA can be detected with sufficient precision.
Original language | English |
---|---|
Publisher | SCK CEN |
Number of pages | 11 |
State | Published - Apr 1964 |
Publication series
Name | SCK CEN Reports |
---|---|
Publisher | SCK CEN |
No. | BLG-343 |