Molecular investigation of radiation resistant cyanobacterium Arthrospira sp. PCC8005

Hanène Badri; Paul Janssen; Felice Mastroleo

Molecular investigation of radiation resistant cyanobacterium Arthrospira sp. PCC8005

Hanène Badri, Paul Janssen, Felice Mastroleo

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Abstract

The cyanobacterium Arthrospira sp.PCC 8005 was selected by European Space Agency (ESA) for producing oxygen and food during future long-duration manned space missions, as part of the bioregenerative life support system 'MELiSSA'. For this task, it is essential that Arthrospira sp. PCC 8005 continues to produce oxygen and conserves high nutritive value while exposed to cosmic radiation in space. Our study showed that the Arthrospira cells are resistant to high doses of ionizing radiation, including both electromagnetic and particle irradiation. The live planktonic cells of Arthrospira sp. PCC 8005 are able to survive and fully recover their photosynthetic growth after exposure to doses of 6400 Gy of gamma irradiation, and 1000 and 2000 Gy of He and Fe particle radiation. Using a newly designed microarray chip and an optimized RNA extraction and protein analysis procedure, the molecular response of Arthrospira to high doses of gamma rays was investigated. These results, confirm that Arthrospira sp. PCC 8005 is valuable candidate for biotechnological applications in environments exposed to ionizing radiation, in space and on Earth.

Original language	English
Awarding Institution	Université de Mons
Supervisors/Advisors	Leys, Natalie, Supervisor Wattiez, Ruddy, Supervisor, External person
Place of Publication	Mons, Belgium
Publisher	UMONS - Université de Mons
State	Published - 23 Jan 2015

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Molecular investigation of radiation resistant cyanobacterium Arthrospira sp. PCC8005 - 2015_Badri_PhDthesisFinal published version, 7.48 MBLicense: Unspecified

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title = "Molecular investigation of radiation resistant cyanobacterium Arthrospira sp. PCC8005",

abstract = "The cyanobacterium Arthrospira sp.PCC 8005 was selected by European Space Agency (ESA) for producing oxygen and food during future long-duration manned space missions, as part of the bioregenerative life support system 'MELiSSA'. For this task, it is essential that Arthrospira sp. PCC 8005 continues to produce oxygen and conserves high nutritive value while exposed to cosmic radiation in space. Our study showed that the Arthrospira cells are resistant to high doses of ionizing radiation, including both electromagnetic and particle irradiation. The live planktonic cells of Arthrospira sp. PCC 8005 are able to survive and fully recover their photosynthetic growth after exposure to doses of 6400 Gy of gamma irradiation, and 1000 and 2000 Gy of He and Fe particle radiation. Using a newly designed microarray chip and an optimized RNA extraction and protein analysis procedure, the molecular response of Arthrospira to high doses of gamma rays was investigated. These results, confirm that Arthrospira sp. PCC 8005 is valuable candidate for biotechnological applications in environments exposed to ionizing radiation, in space and on Earth.",

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TY - BOOK

T1 - Molecular investigation of radiation resistant cyanobacterium Arthrospira sp. PCC8005

AU - Badri, Hanène

A2 - Janssen, Paul

A2 - Mastroleo, Felice

N1 - Score=30

PY - 2015/1/23

Y1 - 2015/1/23

N2 - The cyanobacterium Arthrospira sp.PCC 8005 was selected by European Space Agency (ESA) for producing oxygen and food during future long-duration manned space missions, as part of the bioregenerative life support system 'MELiSSA'. For this task, it is essential that Arthrospira sp. PCC 8005 continues to produce oxygen and conserves high nutritive value while exposed to cosmic radiation in space. Our study showed that the Arthrospira cells are resistant to high doses of ionizing radiation, including both electromagnetic and particle irradiation. The live planktonic cells of Arthrospira sp. PCC 8005 are able to survive and fully recover their photosynthetic growth after exposure to doses of 6400 Gy of gamma irradiation, and 1000 and 2000 Gy of He and Fe particle radiation. Using a newly designed microarray chip and an optimized RNA extraction and protein analysis procedure, the molecular response of Arthrospira to high doses of gamma rays was investigated. These results, confirm that Arthrospira sp. PCC 8005 is valuable candidate for biotechnological applications in environments exposed to ionizing radiation, in space and on Earth.

AB - The cyanobacterium Arthrospira sp.PCC 8005 was selected by European Space Agency (ESA) for producing oxygen and food during future long-duration manned space missions, as part of the bioregenerative life support system 'MELiSSA'. For this task, it is essential that Arthrospira sp. PCC 8005 continues to produce oxygen and conserves high nutritive value while exposed to cosmic radiation in space. Our study showed that the Arthrospira cells are resistant to high doses of ionizing radiation, including both electromagnetic and particle irradiation. The live planktonic cells of Arthrospira sp. PCC 8005 are able to survive and fully recover their photosynthetic growth after exposure to doses of 6400 Gy of gamma irradiation, and 1000 and 2000 Gy of He and Fe particle radiation. Using a newly designed microarray chip and an optimized RNA extraction and protein analysis procedure, the molecular response of Arthrospira to high doses of gamma rays was investigated. These results, confirm that Arthrospira sp. PCC 8005 is valuable candidate for biotechnological applications in environments exposed to ionizing radiation, in space and on Earth.

KW - MELiSSA

KW - Arthrospira

KW - radiation

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_140051

M3 - Doctoral thesis

PB - UMONS - Université de Mons

CY - Mons, Belgium

ER -