Molecular Structure and Metal-binding Properties of the Periplasmic CopK Protein Expressed in Cupriavidus metallidurans CH34 During Copper Challenge

Beate Bersch, Adrien Favier, Paul Schanda, Sébastien van Aelst, Tatiana Vallaeys, Jacques Covès, Max Mergeay, Ruddy Wattiez, Rob Van Houdt

    Research outputpeer-review

    Abstract

    The copK gene is localized on the pMOL30 plasmid of Cupriavidus metallidurans CH34 within the complex cop cluster of genes, for which 21 genes have been identified. The expression of the corresponding periplasmic CopK protein is strongly upregulated in the presence of copper, leading to a high periplasmic accumulation. The structure and metal-binding properties of CopK were investigated by NMR and mass spectrometry.
    Original languageEnglish
    Pages (from-to)386-403
    JournalJournal of Molecular Biology
    Volume380
    Issue number2
    DOIs
    StatePublished - 4 Jul 2008

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