Monitoring Poly(3-hydroxybutyrate) Production in Cupriavidus necator DSM 428 (H16) with Raman Spectroscopy

JD Gelder, D. Willemse-Erix, MJ Scholtes, JI Sanchez, K. Maquelin, P. Vandenabeele, Patrick De Boever, GJ Puppels, L. Moens, PD Vos

    Research outputpeer-review


    This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm-1 appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.
    Original languageEnglish
    Pages (from-to)2155-2160
    JournalAnalytical chemistry
    Issue number6
    StatePublished - 15 Mar 2008

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