Nucleotide sequence of the envelope gene of radiation leukemia virus

J. Merregaert; J. M. Nuyten; M. Janowski

doi:10.1016/0042-6822(85)90286-7

Nucleotide sequence of the envelope gene of radiation leukemia virus

J. Merregaert, J. M. Nuyten, M. Janowski

Research output › peer-review

Abstract

The nucleotide sequence and the predicted amino acid sequence of the envelope gene (env) of RadLV/VL₃ (T⁺L⁺) has been determined. RadLV/VL₃ (T⁺L⁺) is a highly thymotropic (T⁺) and leukemogenic (L⁺) murine recombinant retrovirus continuously produced at high titer by BL/VL₃ cells, a line established in culture from a radiation leukemia virus (RadLV)-induced C57BL/Ka mouse thymic lymphoma. The envelope gene is strikingly similar (92% homologous) to that encoded by the ecotropic Akv-MuLV. Among the differences, 134 scattered, although unevenly distributed point mutations (6.4%), two 9-bp deletions as well as a 6-bp insertion were observed (which result in 49 amino acid changes in the env gene of RadLV/VL₃ (T⁺L⁺)).

Original language	English
Pages (from-to)	457-467
Number of pages	11
Journal	Virology
Volume	144
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(85)90286-7
State	Published - 30 Jul 1985

Funding

This work was performed with the financial support of the European Atomic Energy Commission (Contract BIOD-379-81-B), the Belgian Funds for Scientific Medical Research, and the European Late Effects Project Group. We thank C. Haesen for typing this manuscript and Fr. Schepens for the photographicw ork. The authors thank Dr. R. Hooghe, Dr. F. S. Pedersen, and Dr. M. Etzerodt for helpful discussions.W e thank Dr. R. R. O’Neill for providing the NZB-xenotropic env-gene sequenceb efore publication.

Funders	Funder number
Not added	BIOD-379-81-B

ASJC Scopus subject areas

Virology

Access to Document

10.1016/0042-6822(85)90286-7

Cite this

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title = "Nucleotide sequence of the envelope gene of radiation leukemia virus",

abstract = "The nucleotide sequence and the predicted amino acid sequence of the envelope gene (env) of RadLV/VL3 (T+L+) has been determined. RadLV/VL3 (T+L+) is a highly thymotropic (T+) and leukemogenic (L+) murine recombinant retrovirus continuously produced at high titer by BL/VL3 cells, a line established in culture from a radiation leukemia virus (RadLV)-induced C57BL/Ka mouse thymic lymphoma. The envelope gene is strikingly similar (92% homologous) to that encoded by the ecotropic Akv-MuLV. Among the differences, 134 scattered, although unevenly distributed point mutations (6.4%), two 9-bp deletions as well as a 6-bp insertion were observed (which result in 49 amino acid changes in the env gene of RadLV/VL3 (T+L+)).",

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N2 - The nucleotide sequence and the predicted amino acid sequence of the envelope gene (env) of RadLV/VL3 (T+L+) has been determined. RadLV/VL3 (T+L+) is a highly thymotropic (T+) and leukemogenic (L+) murine recombinant retrovirus continuously produced at high titer by BL/VL3 cells, a line established in culture from a radiation leukemia virus (RadLV)-induced C57BL/Ka mouse thymic lymphoma. The envelope gene is strikingly similar (92% homologous) to that encoded by the ecotropic Akv-MuLV. Among the differences, 134 scattered, although unevenly distributed point mutations (6.4%), two 9-bp deletions as well as a 6-bp insertion were observed (which result in 49 amino acid changes in the env gene of RadLV/VL3 (T+L+)).

AB - The nucleotide sequence and the predicted amino acid sequence of the envelope gene (env) of RadLV/VL3 (T+L+) has been determined. RadLV/VL3 (T+L+) is a highly thymotropic (T+) and leukemogenic (L+) murine recombinant retrovirus continuously produced at high titer by BL/VL3 cells, a line established in culture from a radiation leukemia virus (RadLV)-induced C57BL/Ka mouse thymic lymphoma. The envelope gene is strikingly similar (92% homologous) to that encoded by the ecotropic Akv-MuLV. Among the differences, 134 scattered, although unevenly distributed point mutations (6.4%), two 9-bp deletions as well as a 6-bp insertion were observed (which result in 49 amino acid changes in the env gene of RadLV/VL3 (T+L+)).

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