Observation of dividing spermatocytes for chromosome aberrations induced in mouse spermatologonia by chemical mutagens

A. Leonard; G. Linden

doi:10.1016/0027-5107(72)90160-1

Observation of dividing spermatocytes for chromosome aberrations induced in mouse spermatologonia by chemical mutagens

A. Leonard, G. Linden

Research output › peer-review

Abstract

The ability to produce chromosome aberrations in mouse spermatogonia was tested for six chemical compounds: 2,3,5-tris(ethylenimino)-1,4-benzoquinone (Trenimon); N,N-bis(2-chloroethyl)-N′,O-propylenephosphoric acid ester diamide (Endoxan); methyl methanesulfonate (MMS); propyl methanesulfonate (PMS); isopropyl methanesulfate (IMS); and 1,4-bis(methanesulfonoxy)butane (Myleran). The following dose levels were used: 0.2 mg of Trenimon per kg body weight; 200 mg of Endoxan per kg; 50 mg of MMS per kg; 600 mg of PMS per kg; 75 mg of IMS per kg and 16.0 mg of Myleran per kg. Treated and control animals were killed 100 days after treatment, and their dividing spermatocytes were analyzed for the presence of chromosome aberrations such as reciprocal translocations, fragments and X-Y or autosomal univalents induced in spermatogonial stages. From this analysis it was concluded that those chemical mutagens are ineffective in the production in spermatogonial cells of chromosome anomalies that are detectable in the dividing spermatocytes.

Original language	English
Pages (from-to)	297-300
Number of pages	4
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/0027-5107(72)90160-1
State	Published - Nov 1972

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/0027-5107(72)90160-1

Cite this

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title = "Observation of dividing spermatocytes for chromosome aberrations induced in mouse spermatologonia by chemical mutagens",

abstract = "The ability to produce chromosome aberrations in mouse spermatogonia was tested for six chemical compounds: 2,3,5-tris(ethylenimino)-1,4-benzoquinone (Trenimon); N,N-bis(2-chloroethyl)-N′,O-propylenephosphoric acid ester diamide (Endoxan); methyl methanesulfonate (MMS); propyl methanesulfonate (PMS); isopropyl methanesulfate (IMS); and 1,4-bis(methanesulfonoxy)butane (Myleran). The following dose levels were used: 0.2 mg of Trenimon per kg body weight; 200 mg of Endoxan per kg; 50 mg of MMS per kg; 600 mg of PMS per kg; 75 mg of IMS per kg and 16.0 mg of Myleran per kg. Treated and control animals were killed 100 days after treatment, and their dividing spermatocytes were analyzed for the presence of chromosome aberrations such as reciprocal translocations, fragments and X-Y or autosomal univalents induced in spermatogonial stages. From this analysis it was concluded that those chemical mutagens are ineffective in the production in spermatogonial cells of chromosome anomalies that are detectable in the dividing spermatocytes.",

author = "A. Leonard and G. Linden",

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AU - Linden, G.

PY - 1972/11

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N2 - The ability to produce chromosome aberrations in mouse spermatogonia was tested for six chemical compounds: 2,3,5-tris(ethylenimino)-1,4-benzoquinone (Trenimon); N,N-bis(2-chloroethyl)-N′,O-propylenephosphoric acid ester diamide (Endoxan); methyl methanesulfonate (MMS); propyl methanesulfonate (PMS); isopropyl methanesulfate (IMS); and 1,4-bis(methanesulfonoxy)butane (Myleran). The following dose levels were used: 0.2 mg of Trenimon per kg body weight; 200 mg of Endoxan per kg; 50 mg of MMS per kg; 600 mg of PMS per kg; 75 mg of IMS per kg and 16.0 mg of Myleran per kg. Treated and control animals were killed 100 days after treatment, and their dividing spermatocytes were analyzed for the presence of chromosome aberrations such as reciprocal translocations, fragments and X-Y or autosomal univalents induced in spermatogonial stages. From this analysis it was concluded that those chemical mutagens are ineffective in the production in spermatogonial cells of chromosome anomalies that are detectable in the dividing spermatocytes.

AB - The ability to produce chromosome aberrations in mouse spermatogonia was tested for six chemical compounds: 2,3,5-tris(ethylenimino)-1,4-benzoquinone (Trenimon); N,N-bis(2-chloroethyl)-N′,O-propylenephosphoric acid ester diamide (Endoxan); methyl methanesulfonate (MMS); propyl methanesulfonate (PMS); isopropyl methanesulfate (IMS); and 1,4-bis(methanesulfonoxy)butane (Myleran). The following dose levels were used: 0.2 mg of Trenimon per kg body weight; 200 mg of Endoxan per kg; 50 mg of MMS per kg; 600 mg of PMS per kg; 75 mg of IMS per kg and 16.0 mg of Myleran per kg. Treated and control animals were killed 100 days after treatment, and their dividing spermatocytes were analyzed for the presence of chromosome aberrations such as reciprocal translocations, fragments and X-Y or autosomal univalents induced in spermatogonial stages. From this analysis it was concluded that those chemical mutagens are ineffective in the production in spermatogonial cells of chromosome anomalies that are detectable in the dividing spermatocytes.

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