Abstract
The kinetics of incorporation of labelled precursors into the DNA of isolated rat liver mitochondria has been studied after X-irradiation in vitro. Incorporation of3H-TTP was slightly inhibited during the initial period, followed by an increase of about 25 % of the specific activity. Incorporations of3H-TdR and3H-BUdR were also stimulated by irradiation of mitochondria, although the amount of incorporated precursor was lower than that of3H-TTP. This radiation-induced incorporation of precursors was abolished by the addition of ethidium bromide or EDTA. Degradation of mt-DNA, indicated by the decrease of specific radioactivity, was observed after irradiation of pre-labelled mitochondria; in presence of EDTA the degradation was not detected. These results were discussed from the viewpoint of the repair synthesis of mitochondrial DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 243-252 |
| Number of pages | 10 |
| Journal | Biophysik |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1973 |
| Externally published | Yes |
ASJC Scopus subject areas
- Radiation
- Biophysics
- Environmental Chemistry
- Radiology Nuclear Medicine and imaging
- Environmental Science (miscellaneous)
- General Environmental Science
- Agricultural and Biological Sciences (miscellaneous)
- Physics and Astronomy (miscellaneous)