Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

Max Mergeay, Catherine Tricot, Sébastien van Aelst, Ruddy Wattiez, Victor Stalon, Johan Wouters, Sébastien Monchy, Rafi Benotmane

    Research outputpeer-review


    CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A ˚ , and diffract to 2.2 A ˚ resolution using synchrotron radiation.
    Original languageEnglish
    Pages (from-to)825-827
    JournalActa Crystallographica Section F
    Issue number9
    StatePublished - Aug 2005

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