Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

Max Mergeay; Catherine Tricot; Sébastien van Aelst; Ruddy Wattiez; Victor Stalon; Johan Wouters; Sébastien Monchy; Rafi Benotmane

doi:10.1107/S174430910502316X

Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

Max Mergeay, Catherine Tricot, Sébastien van Aelst, Ruddy Wattiez, Victor Stalon, Johan Wouters, Sébastien Monchy, Rafi Benotmane

Research output › peer-review

Abstract

CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A ˚ , and diffract to 2.2 A ˚ resolution using synchrotron radiation.

Original language	English
Pages (from-to)	825-827
Journal	Acta Crystallographica Section F
Volume	61
Issue number	9
DOIs	https://doi.org/10.1107/S174430910502316X
State	Published - Aug 2005

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Cite this

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title = "Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans",

abstract = "CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A ˚ , and diffract to 2.2 A ˚ resolution using synchrotron radiation.",

keywords = "crystallography, copper resistance, protein, Cupriavidus metallidurans",

author = "Max Mergeay and Catherine Tricot and {van Aelst}, S{\'e}bastien and Ruddy Wattiez and Victor Stalon and Johan Wouters and S{\'e}bastien Monchy and Rafi Benotmane",

note = "Score = 10",

year = "2005",

month = aug,

doi = "10.1107/S174430910502316X",

language = "English",

volume = "61",

pages = "825--827",

journal = "Acta Crystallographica Section F",

issn = "2053-230X",

publisher = "IUCr - International Union of Crystallography",

number = "9",

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TY - JOUR

T1 - Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

AU - Mergeay, Max

AU - Tricot, Catherine

AU - van Aelst, Sébastien

AU - Wattiez, Ruddy

AU - Stalon, Victor

AU - Wouters, Johan

A2 - Monchy, Sébastien

A2 - Benotmane, Rafi

N1 - Score = 10

PY - 2005/8

Y1 - 2005/8

N2 - CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A ˚ , and diffract to 2.2 A ˚ resolution using synchrotron radiation.

AB - CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A ˚ , and diffract to 2.2 A ˚ resolution using synchrotron radiation.

KW - crystallography

KW - copper resistance

KW - protein

KW - Cupriavidus metallidurans

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_27232

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U2 - 10.1107/S174430910502316X

DO - 10.1107/S174430910502316X

M3 - Article

SN - 2053-230X

VL - 61

SP - 825

EP - 827

JO - Acta Crystallographica Section F

JF - Acta Crystallographica Section F

IS - 9

ER -