Phenotypic properties of Bacillus subtilis mutants defective in recombination and repair functions

Igor Samojlenko; Nigel Harford; Max Mergeay

doi:10.1007/BF00269085

Phenotypic properties of Bacillus subtilis mutants defective in recombination and repair functions

Igor Samojlenko, Nigel Harford, Max Mergeay

Research output › peer-review

Abstract

Mutants representing four loci (recB, recD, recE, recF) specifying recombination-repair functions in Bacillus subtilis have been characterized in terms of their response in transformation, to transfection by SPP1 or SP50 phage DNA and their sensitivity to X-irradiation. Each of the four mutants is more sensitive to X-rays than wild type and shows a reduced ability to transform as measured by the uptake and expression of ³H-labelled DNA. Transfection is reduced in recB and recF strains but the recD and recE mutants appear to retain a high level of transfectability for SP50 phage DNA. The recB and recE mutants retain some capacity for repair of X-ray induced single-strand breaks as judged by alkaline sucrose density gradient analysis. The recF and recD strains show no apparent ability to repair single-strand breaks after irradiation.

Original language	English
Pages (from-to)	143-152
Number of pages	10
Journal	MGG Molecular & General Genetics
Volume	130
Issue number	2
DOIs	https://doi.org/10.1007/BF00269085
State	Published - Jun 1974

ASJC Scopus subject areas

Genetics

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10.1007/BF00269085

Cite this

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title = "Phenotypic properties of Bacillus subtilis mutants defective in recombination and repair functions",

abstract = "Mutants representing four loci (recB, recD, recE, recF) specifying recombination-repair functions in Bacillus subtilis have been characterized in terms of their response in transformation, to transfection by SPP1 or SP50 phage DNA and their sensitivity to X-irradiation. Each of the four mutants is more sensitive to X-rays than wild type and shows a reduced ability to transform as measured by the uptake and expression of 3H-labelled DNA. Transfection is reduced in recB and recF strains but the recD and recE mutants appear to retain a high level of transfectability for SP50 phage DNA. The recB and recE mutants retain some capacity for repair of X-ray induced single-strand breaks as judged by alkaline sucrose density gradient analysis. The recF and recD strains show no apparent ability to repair single-strand breaks after irradiation.",

author = "Igor Samojlenko and Nigel Harford and Max Mergeay",

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T1 - Phenotypic properties of Bacillus subtilis mutants defective in recombination and repair functions

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AU - Harford, Nigel

AU - Mergeay, Max

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AB - Mutants representing four loci (recB, recD, recE, recF) specifying recombination-repair functions in Bacillus subtilis have been characterized in terms of their response in transformation, to transfection by SPP1 or SP50 phage DNA and their sensitivity to X-irradiation. Each of the four mutants is more sensitive to X-rays than wild type and shows a reduced ability to transform as measured by the uptake and expression of 3H-labelled DNA. Transfection is reduced in recB and recF strains but the recD and recE mutants appear to retain a high level of transfectability for SP50 phage DNA. The recB and recE mutants retain some capacity for repair of X-ray induced single-strand breaks as judged by alkaline sucrose density gradient analysis. The recF and recD strains show no apparent ability to repair single-strand breaks after irradiation.

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