Physiological and genomic analysis of Limnospira sp. AlgLouSa11 isolated from Tamanrasset, Algeria

Safia Lounici, Lynda Boutekrabt, Amel Doumandji, Selma Salhi, Rob Van Houdt, Natalie Leys

Research outputpeer-review


Cyanobacteria designated as Spirulina, Arthrospira and Limnospira are worldwide well-known food and food supplement sources. Despite their long history of use for human consumption, many indigenous strains remain unstudied. Here we performed a genomic and physiological characterization of AlgLouSa11, an Algerian strain isolated from the Taguemart region in Tamanrasset. Its genome was sequenced and compared (via ANI and PaSit4) to 21 genomes of the Arthrospira and Limnospira genera, indicating that AlgLouSa11 belonged to the Limnospira genus, with Limnospira fusiformis KN01 being the closest relative (99.3 %). Growth was scored in three different culture media (Hiri, Zarrouk, and BG-11), with the highest cell density, 4.76 × 105 cells/ml after 11 days, obtained for Hiri medium. The optimal growth temperature was 34°C, with automatic agitation producing the highest yields (5.90 ± 0.25 g/l). The optimal growth pH was nine, with growth decreasing with increasing alkalinity. Finally, exploring the genome of Limnospira sp. AlgLouSa11 showed the presence of coding sequences involved in the biosynthesis of secondary metabolites such as cyanobactin, terpenes and lanthipeptides.

Original languageEnglish
Article number103569
Number of pages9
JournalAlgal Research
StatePublished - Jul 2024

ASJC Scopus subject areas

  • Agronomy and Crop Science

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