Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12

Max Mergeay; Daniel Gigot; Jacques Beckmann; Nicolas Glansdorff; André Piérard

doi:10.1007/BF00332706

Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12

Max Mergeay, Daniel Gigot, Jacques Beckmann, Nicolas Glansdorff, André Piérard

Research output › peer-review

Abstract

76 mutants have been isolated in which the function of the single carbamoylphosphate synthetase of Escherichia coli K 12 is affected. A wide variety of phenotypes have been observed among these mutants, the most typical ones being: requirement for arginine and uracil, arginineless behaviour, sensitivity towards arginine and sensitivity towards uracil. The mutations have been localized by reciprocal transduction and deletion mapping; all are clustered in the same locus, car. The study of carbamoylphosphate synthesizing activities of these mutants and the combination of car mutations in various in vivo as well as in vitro complementation tests lead to the conclusion that car contains two genes: carA, covering the left part of the locus and coding for the "glutamine subunit" of the enzyme; carB, to the right, governing the synthesis of the heavy subunit of the enzyme.

Original language	English
Pages (from-to)	299-316
Number of pages	18
Journal	MGG Molecular & General Genetics
Volume	133
Issue number	4
DOIs	https://doi.org/10.1007/BF00332706
State	Published - Dec 1974

ASJC Scopus subject areas

Genetics

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10.1007/BF00332706

Cite this

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title = "Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12",

abstract = "76 mutants have been isolated in which the function of the single carbamoylphosphate synthetase of Escherichia coli K 12 is affected. A wide variety of phenotypes have been observed among these mutants, the most typical ones being: requirement for arginine and uracil, arginineless behaviour, sensitivity towards arginine and sensitivity towards uracil. The mutations have been localized by reciprocal transduction and deletion mapping; all are clustered in the same locus, car. The study of carbamoylphosphate synthesizing activities of these mutants and the combination of car mutations in various in vivo as well as in vitro complementation tests lead to the conclusion that car contains two genes: carA, covering the left part of the locus and coding for the {"}glutamine subunit{"} of the enzyme; carB, to the right, governing the synthesis of the heavy subunit of the enzyme.",

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AU - Glansdorff, Nicolas

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AB - 76 mutants have been isolated in which the function of the single carbamoylphosphate synthetase of Escherichia coli K 12 is affected. A wide variety of phenotypes have been observed among these mutants, the most typical ones being: requirement for arginine and uracil, arginineless behaviour, sensitivity towards arginine and sensitivity towards uracil. The mutations have been localized by reciprocal transduction and deletion mapping; all are clustered in the same locus, car. The study of carbamoylphosphate synthesizing activities of these mutants and the combination of car mutations in various in vivo as well as in vitro complementation tests lead to the conclusion that car contains two genes: carA, covering the left part of the locus and coding for the "glutamine subunit" of the enzyme; carB, to the right, governing the synthesis of the heavy subunit of the enzyme.

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