Preparation, properties and storage of nucleic acids

Lucien Ledoux, C. Davila, Raoul Huart, Pol Charles

    Research outputpeer-review


    A method has been developed for the fractionation of ribo- and deoxyribonucleic acid on DEAE-cellulose paper (strips or centrifuged pulp). In the case of DNA, specific chromatographic profiles were obtained on strips for quantities of about one microgram.
    Six to twelve fractions can thus be easily separated in a very short space of time.
    Because of the ease and reproducibility of the method, a large number of samples can be analyzed at the same time. By means of this method, we studied various separation techniques now being used in the biochemistry of nucleic acids, together with the effects of ageing, denaturing due to heat, enzymatic hydrolysis and ultra-sounds. About thirty different nucleic acid preparations labelled with 14C and/or 3H were made from various starting materials. By the use of bacteria mutants, we were able to obtain nucleic acids having a very high specific activity, and we are now studying spontaneous radiolysis of them.
    The results obtained will be discussed, together with the possibilities of using the method in biology.
    Original languageEnglish
    PublisherSCK CEN
    Number of pages14
    StatePublished - Nov 1963

    Publication series

    NameSCK CEN Reports
    PublisherSCK CEN

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