Preparation, properties and storage of nucleic acids

Lucien Ledoux; C. Davila; Raoul Huart; Pol Charles

Preparation, properties and storage of nucleic acids

Lucien Ledoux, C. Davila, Raoul Huart, Pol Charles

Research output › peer-review

Abstract

A method has been developed for the fractionation of ribo- and deoxyribonucleic acid on DEAE-cellulose paper (strips or centrifuged pulp). In the case of DNA, specific chromatographic profiles were obtained on strips for quantities of about one microgram.
Six to twelve fractions can thus be easily separated in a very short space of time.
Because of the ease and reproducibility of the method, a large number of samples can be analyzed at the same time. By means of this method, we studied various separation techniques now being used in the biochemistry of nucleic acids, together with the effects of ageing, denaturing due to heat, enzymatic hydrolysis and ultra-sounds. About thirty different nucleic acid preparations labelled with 14C and/or 3H were made from various starting materials. By the use of bacteria mutants, we were able to obtain nucleic acids having a very high specific activity, and we are now studying spontaneous radiolysis of them.
The results obtained will be discussed, together with the possibilities of using the method in biology.

Original language	English
Publisher	SCK CEN
Number of pages	14
State	Published - Nov 1963

Publication series

Name	SCK CEN Reports
Publisher	SCK CEN
No.	BLG-325

Cite this

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title = "Preparation, properties and storage of nucleic acids",

abstract = "A method has been developed for the fractionation of ribo- and deoxyribonucleic acid on DEAE-cellulose paper (strips or centrifuged pulp). In the case of DNA, specific chromatographic profiles were obtained on strips for quantities of about one microgram.Six to twelve fractions can thus be easily separated in a very short space of time.Because of the ease and reproducibility of the method, a large number of samples can be analyzed at the same time. By means of this method, we studied various separation techniques now being used in the biochemistry of nucleic acids, together with the effects of ageing, denaturing due to heat, enzymatic hydrolysis and ultra-sounds. About thirty different nucleic acid preparations labelled with 14C and/or 3H were made from various starting materials. By the use of bacteria mutants, we were able to obtain nucleic acids having a very high specific activity, and we are now studying spontaneous radiolysis of them. The results obtained will be discussed, together with the possibilities of using the method in biology.",

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AU - Ledoux, Lucien

AU - Davila, C.

AU - Huart, Raoul

AU - Charles, Pol

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N2 - A method has been developed for the fractionation of ribo- and deoxyribonucleic acid on DEAE-cellulose paper (strips or centrifuged pulp). In the case of DNA, specific chromatographic profiles were obtained on strips for quantities of about one microgram.Six to twelve fractions can thus be easily separated in a very short space of time.Because of the ease and reproducibility of the method, a large number of samples can be analyzed at the same time. By means of this method, we studied various separation techniques now being used in the biochemistry of nucleic acids, together with the effects of ageing, denaturing due to heat, enzymatic hydrolysis and ultra-sounds. About thirty different nucleic acid preparations labelled with 14C and/or 3H were made from various starting materials. By the use of bacteria mutants, we were able to obtain nucleic acids having a very high specific activity, and we are now studying spontaneous radiolysis of them. The results obtained will be discussed, together with the possibilities of using the method in biology.

AB - A method has been developed for the fractionation of ribo- and deoxyribonucleic acid on DEAE-cellulose paper (strips or centrifuged pulp). In the case of DNA, specific chromatographic profiles were obtained on strips for quantities of about one microgram.Six to twelve fractions can thus be easily separated in a very short space of time.Because of the ease and reproducibility of the method, a large number of samples can be analyzed at the same time. By means of this method, we studied various separation techniques now being used in the biochemistry of nucleic acids, together with the effects of ageing, denaturing due to heat, enzymatic hydrolysis and ultra-sounds. About thirty different nucleic acid preparations labelled with 14C and/or 3H were made from various starting materials. By the use of bacteria mutants, we were able to obtain nucleic acids having a very high specific activity, and we are now studying spontaneous radiolysis of them. The results obtained will be discussed, together with the possibilities of using the method in biology.

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