Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34

Caroline Rosier, Natalie Leys, Céline Henoumont, Max Mergeay, Ruddy Wattiez, Rob Van Houdt

    Research outputpeer-review


    Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteriaceae family, genome analyses revealed that bacterial that possess Acx are associated to the soil such as Cupriavidus metallidurans strain CH34. Acx of CH34 forms a heterohexameric complex α2β2γ2 and can only carboxylate acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.
    Original languageEnglish
    Pages (from-to)4516-4518
    JournalApplied and Environmental Microbiology
    Issue number12
    StatePublished - Jun 2012

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