TY - JOUR
T1 - Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS)
AU - Radstake, Wilhelmina E.
AU - Parisi, Alessio
AU - Ferreira da Silva Miranda, Silvana
AU - Gautam, Kiran
AU - Vermeesen, Randy
AU - Rehnberg, Emil
AU - Tabury, Kevin
AU - Coppes, Rob
AU - van Goethem, Marc Jan
AU - Brandenburg, Sytze
AU - Weber, Ulrich
AU - Fournier, Claudia
AU - Durante, Marco
AU - Baselet, Bjorn
AU - Baatout, Sarah
N1 - Score=10
Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Without the protective shielding of Earth’s atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities.
AB - Without the protective shielding of Earth’s atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities.
KW - Carbon ions
KW - Cortisol
KW - DNA damage
KW - DNA repair
KW - Fibroblast
KW - Ionizing radiation
KW - Iron ions
KW - Protons
UR - http://www.scopus.com/inward/record.url?scp=85192141693&partnerID=8YFLogxK
U2 - 10.1038/s41598-024-60912-y
DO - 10.1038/s41598-024-60912-y
M3 - Article
C2 - 38710823
AN - SCOPUS:85192141693
SN - 2045-2322
VL - 14
SP - 1
EP - 15
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 10400
ER -